Isolation effectively prevents the transmission of hepatitis C virus in the hemodialysis unit

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Title:	Isolation effectively prevents the transmission of hepatitis C virus in the hemodialysis unit
Authors:	Yang, CS;Chang, HH;Chou, CC;Peng, SJ
Contributors:	中國醫藥大學;Cathay Gen Hosp, Div Nephrol, Sect 4, Dept Internal Med, Taipei, Taiwan;Taipei Med Univ, Cathay Gen Hosp, Div Nephrol, Dept Internal Med, Taipei, Taiwan;China Med Coll, Taichung, Taiwan
Date:	2003
Issue Date:	2010-09-24 13:39:36 (UTC+8)
Publisher:	EXCERPTA MEDICA ASIA LTD
Abstract:	A nonradioactive, sensitive, rapid, and specific method for the determination of methionine adenosyltransferase activity has been established. In this method, the methyl group of S-adenosyl-L-methionine was enzymatically transferred to esculetin with the aid of catechol-O-methyltransferase and then the resulting scopoletin was extracted with n-hexane:ethyl acetate (7:3, v/v) and measured by high-performance liquid chromatography with Si 60 column and fluorometric detection with excitation and emission wavelengths at 347 and 415 nm, respectively. The detection limit for scopoletin was about 100 fmol. Using this method to determine MAT activity in HL-60 cells required only about 2.5 mug of protein and the incubation time needed for enzymatic reaction is less than 30 min. The HPLC analysis procedure took only 5 min per sample. The kinetic study showed that MAT in HL-60 cells exhibited negative cooperativity with a Hill coefficient of 0.5. The values of K-m and V-max were 6.1+/-0.3 muM and 135.4+/-1.5 nmol AdoMet formed/mg protein/h, respectively. (C) 2003 Elsevier Science (USA). All rights reserved.
Relation:	JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION 102(2):79-85
Appears in Collections:	[China Medical University] Journal articles

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