The aqueous soluble polyphenolic fraction of Psidium guajava leaves exhibits potent anti-angiogenesis and anti-migration actions on DU145 cells

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Title:	The aqueous soluble polyphenolic fraction of Psidium guajava leaves exhibits potent anti-angiogenesis and anti-migration actions on DU145 cells
Authors:	彭瓊琦(Chiung-Chi Peng);(Chiung-Huei Peng);(Kuan-Chou Chen);(Chiu-Lan Hsieh);(Robert Y. Peng)
Contributors:	健康照護學院物理治療學系
Keywords:	anti-angiogenic;anti-migration;chemopreventive;DU145 cells;Psidium guajava
Date:	2010
Issue Date:	2010-09-23 19:56:10 (UTC+8)
Abstract:	The aqueous extract of Psidium guajava budding leaves (PE) bears an extremely high content of polyphenolic and isoflavonoids. Whether it could be used as an anti-tumor chemopreventive in view of anti-angiogenesis and anti-migration, we performed the assay methods including the MTT assay to examine the cell viability; the ELISA assay to test the expressions of VEGF, IL-6 and IL-8; the western blot analysis to detect TIMP-2; the gelatinolytic zymography to follow the expression of MMPs; the wound scratch assay to examine the migration capability; and the chicken chorioallantoic membrane assay to detect the suppressive angiogenesis. Results indicated that the IC50 of PE for DU145 cells was ∼0.57 mg ml−1. In addition, PE effectively inhibited the expressions of VEGF, IL-6 and IL-8 cytokines, and MMP-2 and MMP-9, and simultaneously activated TIMP-2 and suppressed the cell migration and the angiogenesis. Conclusively, PE potentially possesses a strong anti-DU145 effect. Thus, clinically it owns the potential to be used as an effective adjuvant anti-cancer chemopreventive.
Relation:	Evidence-based Complementary and Alternative Medicine ():
Appears in Collections:	[Department of Physical Therapy, Graduate Institute of Rehabilitation Science] Journal articles

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