Crude Extracts of Solanum lyratum Protect Endothelial Cells Against Oxidized Low-Density Lipoprotein-Induced Injury Via Direct Antioxidant Action

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 健康照護學院 > 物理治療學系暨復健科學碩士班 > 期刊論文 > Item 310903500/28439

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题名:	Crude Extracts of Solanum lyratum Protect Endothelial Cells Against Oxidized Low-Density Lipoprotein-Induced Injury Via Direct Antioxidant Action
作者:	郭薇雯(Wei-Wen Kuo);黃志揚(Chih-Yang Huang);鍾景光(Jing-Gung Chung);楊順發(Shun-Fa Yang);蔡昆霖(Kun-Ling Tsai);邱燦宏(Tsan-Hung Chiu);李信達(Shin-Da Lee);歐秀中(Hsiu-Chung Ou)
贡献者:	健康照護學院物理治療學系
日期:	2009-10
上传时间:	2010-09-23 19:55:28 (UTC+8)
摘要:	"BACKGROUND: Oxidized low-density lipoprotein (oxLDL) is a proatherogenic molecule that accumulates in the vascular wall and contributes to the pathogenesis of vascular dysfunction early in the development of atherosclerosis. The whole plant of Solanum lyratum is a traditional Chinese medicine that has been used for centuries to treat cancer, tumors, and herpes. However, the cellular and molecular mechanisms of its antioxidant effects are still largely unknown. This study tested the hypothesis that Solanum lyratum Thunberg extract (SLE) could block oxLDL-induced endothelial dysfunction in cultured human umbilical vein endothelial cells (HUVECs). Possible mechanisms were explored. METHODS: Antioxidative activities of SLE were assayed by measuring the scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical and the inhibition of copper-mediated or cell-mediated LDL oxidation. Production of reactive oxygen species (ROS) and the expression of adhesion molecules were evaluated in HUVECs after exposure to oxLDL and treatment with SLE. Several apoptotic signaling pathways were investigated. RESULTS: SLE scavenged DPPH and also delayed the kinetics of LDL oxidation in a dose-dependent manner. SLE attenuated the level of oxLDL-induced ROS generation, diminished the expression of endothelial NO synthase (eNOS), and enhanced the expression of adhesion molecules (vascular cellular adhesion molecule-1, E-selectin, and monocyte chemotactic protein-1) and the adherence of monocytic THP-1 cells to HUVECs. OxLDL increased the concentration of intracellular calcium, disturbed the balance of the Bcl-2 protein family, destabilized the mitochondrial membrane potential, increased the amount of cytochrome c released into the cytosol, and increased the activation of caspase 3. These detrimental effects were ameliorated dose-dependently by SLE (P < .05). CONCLUSION: Crude extracts of Solanum lyratum protect against oxLDL-induced injury in endothelial cells by direct antioxidant action. CLINICAL RELEVANCE: Atherosclerosis is a chronic inflammatory disease characterized by lipid-laden lesions within arterialblood vessel walls. Inhibiting the oxidation of low-density lipoprotein may be an effective way to prevent or delay theprogression of atherosclerosis. This study underscores the potential clinical benefits and application of Solanum lyratumextract in controlling oxidized low-density lipoprotein-associated vascular injury and cardiovascular disease."
關聯:	JOURNAL OF VASCULAR SURGERY 50(4):849-860
显示于类别:	[物理治療學系暨復健科學碩士班] 期刊論文

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