Sesamin mitigates inflammation and oxidative stress in endothelial cells exposed to oxidized low-density lipoprotein

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Title:	Sesamin mitigates inflammation and oxidative stress in endothelial cells exposed to oxidized low-density lipoprotein
Authors:	歐秀中(Hsiu-Chung Ou);李文珍(Wen-Jane Lee);吳靜玫(Ching-Mei Wu);李奕德(I-Te Lee);林時逸(Shih-Yi Lin);林麗雲(Li-Yun Lin);蔡昆霖(Kun-Ling Tsai);李信達(Shin-Da Lee);許惠恒(Wayne Huey-Herng Sheu)*
Contributors:	健康照護學院物理治療學系
Keywords:	Endothelium;oxLDL;sesamin;ROS;adhesion molecules;apoptosis
Date:	2009-12
Issue Date:	2010-09-23 19:54:46 (UTC+8)
Abstract:	Sesamin, a lignan from sesame oil, has been shown to have antihypertensive and antioxidative properties. This study examined the effects of sesamin on oxidized low-density lipoprotein (oxLDL)-induced endothelial dysfunction. Oxidative stress was determined by measuring the generation of intracellular reactive oxygen species (ROS) and by measuring the expression levels of superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS). To assess the pro-inflammatory effects of oxLDL, ELISA was used to detect IL-8 expression, endothelin-1 (ET-1) secretion, and nuclear factor-kappaB (NF-kappaB) activation. The expression of adhesion molecules (ICAM-1, VCAM-1, and E-selectin) was examined by flow cytometry. In addition, several apoptotic signaling pathways were also investigated. The data showed that sesamin significantly ameliorated oxLDL-induced ROS generation and SOD-1 inactivation. Sesamin also attenuated the oxLDL-induced activation of NF-kappaB, suggesting that the inhibitory effects of sesamin on IL-8 and ET-1 release, adhesion molecule expression, and the adherence of THP-1 cells were at least partially through the blockade of NF-kappaB activation. Furthermore, sesamin attenuated oxLDL-induced apoptotic features, such as intracellular calcium accumulation and the subsequent collapse of mitochondrial membrane potential, release of cytochrome c, and activation of caspase-3. Results from this study may provide insight into possible molecular mechanisms underlying sesamin's beneficial effects against oxLDL-mediated vascular endothelial dysfunction.
Relation:	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 57(23):11406-11417
Appears in Collections:	[Department of Physical Therapy, Graduate Institute of Rehabilitation Science] Journal articles

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