中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/28390
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    题名: Influence of Collagen I Nanospheres on the Growth and Osteogenic Difference of Rat Bone Marrow Stromal Cells
    作者: (Kuo-Yu Chen)、(Chia-Mei Chung)、(Shyh-Ming Kuo)*、陳悅生(Yueh-Sheng Chen)*、姚俊旭(Chun-Hsu Yao)*
    贡献者: 健康照護學院生物醫學影像暨放射科學學系
    日期: 2009-12
    上传时间: 2010-09-23 19:52:32 (UTC+8)
    摘要: Bone marrow stromal cells (BMSCs) are a promising cell source for bone tissue-engineering application due to their osteogenic differentiation potential. Effective growth and osteoblastic differentiation of BMSCs are required in promoting successful bone formation. In this study, the effect of collagen I nanospheres on the proliferation and osteogenic differentiation of rat BMSCs in vitro was investigated by the addition of collagen I nanospheres directly into the culture medium. The mitochondrial activity of rat BMSCs was measured by colorimetric assay: results showed that collagen I nanospheres promoted rat BMSC proliferation. Osteogenic differentiation of rat BMSCs was qualified via measurements of alkaline phosphatase (ALP) activity and matrix mineralization. Our results demonstrated that there was a striking difference in ALP staining and von Kossa staining. Collagen I nanospheres induced an increase in the number of ALP-positive cells. Bone nodule formation occurred under cells treated with or without collagen I nanospheres, but the extent of mineralization was increased when collagen I nanospheres were present in cultures. These data support that collagen I nanospheres might be suitable for the cultivation of BMSCs for clinical applications. Moreover, the observations suggest that collagen I nanospheres might have a great potential for bone tissue engineering.
    關聯: Journal of Medical and Biological Engineering 29(6):284-289
    显示于类别:[生物醫學影像暨放射科學學系暨碩生班] 期刊論文

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