This study proposes a novel design of an easily stripped bi-layered composite that comprises of an upper layer of soybean protein non-woven fabric, coated with a lower layer, genipin-crosslinked chitosan and bletilla striata herbal extract (GCB) film, as a wound dressing material (GCB/NWF). Preliminary tests revealed the in vitro characteristics of the genipin-crosslinked chitosan (GC) film. Fourier Transform Infrared Spectroscopy (FTIR) has revealed that secondary amide linkages are formed by the reaction between the genipin ester group and the chitosan amino group, yielding a polymeric network structure. Genipin and chitosan were released from the soaked GC film. A cytotoxic test revealed that 20 ppm of the genipin in the culture medium is the concentration threshold, over which it is cytotoxic to production of L929 fibroblasts. However, the chitosan and bletilla striata herbal extract not only were not toxic but also promoted the viability and growth of L929 fibroblasts. Additionally, the GCB film effectively supported cell attachment and growth. The result of the in vivo histological assessment revealed that epithelialization and reconstruction of the wound were achieved by covering it with the GCB/NWF wound dressing material, which was easily stripped from the wound surface without damaging the newly regenerated tissue.
關聯:
Journal of Medical and Biological Engineering 29(2):60-67
