Effects of Garlic Components Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity and 2-Aminofluorene-DNA Adducts in Human Promyelocytic Leukemia Cells

CMUR > College of Chinese Medicine > Graduate Institute of Chinese Medical Science > Journal articles > Item 310903500/2722

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/2722

Title:	Effects of Garlic Components Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity and 2-Aminofluorene-DNA Adducts in Human Promyelocytic Leukemia Cells
Authors:	林昭庚;陳光偉(Guang W.Chen);蘇進成(C.C. Su);洪啟賦(C. F. Hung);楊慶鏘;李昭宏(Jau-Hong Lee);鍾景光*
Contributors:	中醫學院中國醫學研究所;中國附醫院長室
Keywords:	Diallyl Sulfide;Diallyl Disulfide;2-Aminofluorene;HL-60;NAT
Date:	2002-06
Issue Date:	2009-08-20 17:48:07 (UTC+8)
Abstract:	Two components of garlic, diallyl sulfide (DAS) and diallyl disulfide (DADS), inhibited arylamine N-acetyltransferase (NAT) activity and 2-aminofluorene-DNA adduct in human promyelocytic leukemia cells (HL-60). The NAT activity was measured by high performance liquid chromatography assaying for amounts of N-acetyl-2-aminofluorene (2-AAF) and remaining 2-aminofluorene (2-AF). Cellular cytosols and intact cell suspensions were assayed. The inhibition of NAT activity and 2-AF-DNA adduct formation in human leukemia cells by DAS and DADS were dose-dependent and were directly proportional. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax from human leukemia cells in both assays. This is the first report of garlic components affecting human leukemia cell NAT activity and 2-AF-DNA adduct formation.
Relation:	AMERICAN JOURNAL OF CHINESE MEDICINE 30(2):315~325
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

Files in This Item:

File	Size	Format
	0Kb	Unknown	464	View/Open