Aloe-emodin induces apoptosis in T24 human bladder cancer cells through the p53 dependent apoptotic pathway

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Title:	Aloe-emodin induces apoptosis in T24 human bladder cancer cells through the p53 dependent apoptotic pathway
Authors:	林昭庚(Jaung-Geng Lin);陳光偉;李德茂(Te-Mao Li);(Chouh ST);(Tan TW);鍾景光(Jing-Gung Chung)
Contributors:	中醫學院中國醫學研究所;中國附醫院長室
Keywords:	bladder;bladder neoplasms;aloe;emodin;apoptosis
Date:	2006
Issue Date:	2009-08-20 17:48:07 (UTC+8)
Abstract:	Purpose We investigated the anticancer effect of AE (1,8-dihydroy-3-[hydroxymethyl]-anthraquione) in the T24 human bladder cancer cell line (Food Industry Research and Development Institute, Hsinchu, Taiwan) by studying apoptosis regulation. Materials and Methods AE, which is purified from aloe vera leaves, has been reported to have antitumor activity. Cell viability, cell cycle and apoptosis were determined by flow cytometric methods. Levels of cyclins, cyclin-dependent kinase 1 and other enzyme were examined by Western blotting methods. Results AE inhibited cell viability, and induced G2/M arrest and apoptosis in T24 cells. AE increased the levels of Wee1 and cdc25c, and may have led to inhibition of the levels of cyclin-dependent kinase 1 and cyclin B1, which cause G2/M arrest. AE induced p53 expression and was accompanied by the induction of p21 and caspase-3 activation, which was associated with apoptosis. In addition, AE was associated with a marked increase in Fas/APO1 receptor and Bax expression but it inhibited Bcl-2 expression. Conclusions AE induced apoptosis in T24 cells is mediated through the activation of p53, p21, Fas/APO-1, Bax and caspase-3.
Relation:	JOURNAL OF UROLOGY 175(1):343~347
Appears in Collections:	[Graduate Institute of Chinese Medical Science] Journal articles

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