中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/27111
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 29490/55136 (53%)
造訪人次 : 2004516      線上人數 : 406
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於CMUR ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/27111


    題名: Concurrent measurement of unbound genistein in the blood, brain and bile of anesthetized rats using microdialysis and its pharmacokinetic application
    作者: Tsai, TH
    貢獻者: 中醫學院中醫所
    Natl Res Inst Chinese Med, Taipei 112, Taiwan;China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan;Natl Yang Ming Univ, Inst Tradit Med, Taipei 112, Taiwan
    日期: 2005
    上傳時間: 2010-09-20 13:44:14 (UTC+8)
    出版者: ELSEVIER SCIENCE BV
    摘要: Genistein, the major isoflavone in soybeans, has been shown to have a wide range of effects. We used an HPLC-UV combined with microdialysis method to detect unbound genistein in rat blood, brain and bile. Genistein dialysates were eluted with a mobile phase containing acetonitrile-water (40:60, v/v, pH 3.5 adjusted by 0.1% acetic acid). Samples were separated using a phenyl (5 μ m) column maintained at ambient temperature. The UV detector wavelength was set at 259 nm. The flow rate was 1.0 ml/min. The limit of quantitation for genistein was 50 ng/ml. The in vitro recoveries of genistein were 31 ± 1, 13 ± 1 and 59 ± 4% in microdialysis probes of blood, brain and bile, respectively (n = 4). Inter- and intra-assay accuracy and precision of the analysis were less than 10% in the concentration ranges of 0.05-5.0 μ g/ml. A small ratio of genistein penetrates the blood-brain barrier (BBB) and goes through hepatobiliary excretion after genistein administration (10 or 30 mg/kg, i.v.). The brain-to-blood (AUC(brain)/AUC(blood)) and bile-to-blood (AUC(bile)/AUC(blood)) distribution ratios were 0.04 ± 0.01 and 1.85 ± 0.42, respectively for the dosage of genistein 30 mg/kg. After co-administration of cyclosporine, a P-glycoprotein (P-gp) inhibitor, the distribution ratios of genistein in brain and bile were not significantly altered. These results suggest that the BBB penetration and hepatobiliary excretion of genistein may not regulated by P-gp. © 2004 Elsevier B.V. All rights reserved.
    關聯: JOURNAL OF CHROMATOGRAPHY A 1073(1月2日):317-322
    顯示於類別:[中國醫學研究所] 會議論文

    文件中的檔案:

    沒有與此文件相關的檔案.



    在CMUR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

     

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋