Analysis of urinary nucleosides as helper tumor markers in hepatocellular carcinoma diagnosis.

CMUR > College of Chinese Medicine > Graduate Institute of Integrated Medicine > Journal articles > Item 310903500/2705

Please use this identifier to cite or link to this item: http://ir.cmu.edu.tw/ir/handle/310903500/2705

Title:	Analysis of urinary nucleosides as helper tumor markers in hepatocellular carcinoma diagnosis.
Authors:	羅婉瑜(Wan-Yu Lo);鄭隆賓(Long-Bin Jeng);許維儀(Wei-Yi Hsu);林瑋德(Wei-De Lin);林炯璁(Chiung-Tsung Lin);蔡輔仁(Fuu-Jen Tsai)
Contributors:	中醫學院中西醫結合研究所;中國附醫醫學研究部遺傳中心
Date:	2009-09
Issue Date:	2009-08-20 17:46:04 (UTC+8)
Abstract:	Hepatocellular carcinoma (HCC) is a common neoplasm in Taiwan, for which early diagnosis is difficult and the prognosis is usually poor. HCC is usually diagnosed by abdominal sonography and serum alpha-fetoprotein (AFP) detection. Modified nucleosides, regarded as indicators for the whole-body turnover of RNAs, are excreted in abnormal amounts in the urine of patients with malignancies and can serve as tumor markers. We analyzed the excretion patterns of urinary nucleosides from 25 HCC patients and 20 healthy volunteers by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) under optimized conditions. The HPLC/ESI-MS/MS approach with selective reaction monitoring (SRM) allowed for the sensitive determination of nucleosides in human urine samples. The mean levels of the urinary nucleosides adenosine, cytidine, and inosine were significantly higher in HCC patients than healthy volunteers (average of 1.78-, 2.26-, and 1.47-fold, respectively). However, the mean levels of urinary 1-methyladenosine, 3-methylcytidine, uridine, and 2-deoxyguanosine were not significantly different. Combined with the determination of serum AFP levels, the higher levels of urinary adenosine, cytidine, and inosine may be additional diagnosis markers for HCC in Taiwanese patients.
Relation:	RAPID COMMUNICATIONS IN MASS SPECTROMETRY 23(11):1543-1549
Appears in Collections:	[Graduate Institute of Integrated Medicine] Journal articles

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