Mass Spectroscopic Characteristics of Low Molecular Weight Proteins Extracted From Calcium Oxalate Stones: Preliminary Study.

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题名:	Mass Spectroscopic Characteristics of Low Molecular Weight Proteins Extracted From Calcium Oxalate Stones: Preliminary Study.
作者:	陳汶吉(Chen Wen-Chi);賴建成(Chien-Chen Lai);蔡育勳;林維勇(Wei-Yong Lin);蔡輔仁(Fuu-Jen Tsai)
贡献者:	中醫學院中西醫結合研究所;中國附醫泌尿部
关键词:	calcium oxalate;matrix protein;matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF-MS);surface-enhanced laser desorption ionization-time of flight mass spectrometry (SELDI-TOF-MS);leukocyte elastase precursor
日期:	2008-08
上传时间:	2009-08-20 17:45:57 (UTC+8)
摘要:	It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic patterns by mass spectroscopy. A total of 10 stones were surgically removed from patients with urolithiasis. Proteins were extracted from the stones and identified by one-dimensional electrophoresis (sodium dodecyl sulfate buffer [SDS]-polyacrylamide gel electrophoresis [SDS-PAGE]). After in-gel digest, samples were analyzed by the surface-enhanced laser desorption ionization-time of flight (SELDI-TOF) technique. The peptide sequences were analyzed from the data of mass spectroscopy. Proteins were identified from Database Search (SwissProt Protein Database; Swiss Institute of Bioinformatics; http://www.expasy.org/sprot) on a MASCOT server (Matrix Science Ltd.; http://www.matrixscience.com). A total of three bands of proteins (27, 18, and 14 kDa) were identified from SDS-PAGE in each stone sample. A database search (SwissProt) on a MASCOT server revealed that the most frequently seen proteins from band 1 (27 kDa) were leukocyte elastase precursor, cathepsin G precursor, azurocidin precursor, and myeloblastin precursor (EC 3.4.21.76) (leukocyte proteinase 3); band 2 (18 kDa) comprised calgranulin B, eosinophil cationic protein precursor, and lysozyme C precursor; band 3 (14 kDa) showed neutrophil defensin 3 precursor, calgranulin A, calgranulin C, and histone H4. The modifications and deamidations found from the mass pattern of these proteins may provide information for the study of matrix proteins. Various lower molecular weight proteins can be extracted from calcium oxalate stones. The characteristic patterns and their functions of those proteins should be further tested to investigate their roles in stone formation.
關聯:	JOURNAL OF CLINICAL LABORATORY ANALYSIS 22(1):77~85
显示于类别:	[中西醫結合研究所] 期刊論文

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