為了探討補陽還五湯改善腦梗塞與一氧化氮（nitric oxide, NO）調節之關係，我們將Sprague-Dawley大鼠兩側的頸總動脈和右中大腦動脈血流阻斷90分鐘，隨後經24小時的再灌流，建立一個局部腦梗塞的動物模型。評估它們的神經狀態後，將大鼠犧牲並取腦，作成切片，經2,3,5-triphenyl-tetrazolium chloride染色後，計算腦梗塞面積。另外，分別於腦血流阻斷前、阻斷後90min，以及再灌流30min、120min測量周邊血液NO的濃度。結果顯示補陽還五湯0.73克／日和0.15克／日，兩者都能改善大鼠的神經狀態和減少腦梗塞面積。補陽還五湯於再灌流30min時，增加N0的濃度，而於再灌流120min時減少NO的濃度。結論是補陽還五湯改善缺血一再灌流腦梗塞大鼠神經狀態和減少腦梗塞面積與N0調節有關。
In order to investigate Bu-Yang-Huan-Wu Tang (BYHWT) improves cerebral infarct whether or not has relationship to regulation of nitric oxide (NO). We established an animal model of focal cerebral infarct by occluding the bilateral common carotid arteries and the right middle cerebral artery for 90 mm, following by reperfusion 24 hrs in the Sprague-Dawley rats. The rats were killed and brain tissue was stained with 2, 3, 5-triphenyl-tetrazolium chloride, and measured cerebral infarct area after neurological status was observed. In addition, nitricoxide (NO) was measured before and at 90 min after the blood flow occlusion, and at 30 mm and at 120 mm after reperfusion, respectively. Results indicated that Pretreatment with BYWHT 0.73 g/day and 0.15 g/day can improve neurological status and can reduce the area of cerebral infarct in ischemia-reperfusion cerebral infarct rats. The levels of NO increased at 30 mm after reperfusion, whereas the levels of NO decreased at 120 mm after reperfusion in BYWHT-treated rats. In conclusion, BYHWT can improve neurological status and reduced the area of cerebral infarct. This effect of BYHWT has relationship to the regulation of NO in ischemia-reperfusion cerebral infarct rats.
