Introduction: Because of the high incidence and poor
prognosis of gliomas, the development of pre-clinically
effective diagnostic tools is of great importance. The
objective of this study is to validate the use of FDG PET
imaging system for monitoring glioma proliferation in
two rodent models.
Methods: Two kinds of glioblastoma cells (human
DBTRG-05MG and rat RG2 tumor cells) were implanted
intracerebrally to SCID mice and Wistar rats, respectively.
To characterize the optimal scanning time
required for effective detection of brain tumors, dynamic
animal PET were acquired for 1 and 2 h immediately
after intravenous injection of the FDG radiotracer to
mice and rats, respectively. Test animals were then subjected
to serial animal PET scans at day 7, 10, 14, and
17 after tumor cell implantation.
Results: Mouse and rat brain tumors were first detected
by FDG microPET imaging at day 7 and 10 after
tumor implantation, respectively. The smallest tumor
size detectable was 2.5 mm in diameter. The peak
tumor-to-background ratio was observed at 40 min
post-injection in the mouse model and at 90 min postinjection
in the rat study. Both the peak standard uptake
value of FDG and the tumor-to-background ratios were
found to increase as the tumors grew over time.
Conclusion: A FDG PET scan protocol was validated
for detecting and monitoring glioma tumor growth in
both mouse and rat models. Optimal FDG uptake period
required and optimal scanning times for experimental
tests were hence established for future systematic
studies in relevant animal models.
關聯:
Annals of Nuclear Medicine and Sciences 23(2):77~83
