四種苦參屬藥用植物抗氧化活性及其對神經細胞保護作用之研究; The antioxidant activity and neuroprotective effects of four Sophora medical plants in vitro

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題名:	四種苦參屬藥用植物抗氧化活性及其對神經細胞保護作用之研究;The antioxidant activity and neuroprotective effects of four Sophora medical plants in vitro
作者:	賴淑珍;Shu-Chen Lai
貢獻者:	中國醫藥大學中國藥學研究所碩士班
關鍵詞:	氧化壓力;槐花;毛苦參;抗氧化活性;神經細胞保護作用;oxidative stress;S. japonica;S. tomentosa;antioxidant activity;neuroprotective effects
日期:	2005
上傳時間:	2010-01-28 15:08:34 (UTC+8)
摘要:	神經退化性疾病如阿滋海默氏症等，與氧化壓力引起神經細胞損傷有關。因此有學者建議以抗氧化藥物作為神經退化性疾病之預防與治療。本研究蒐集豆科苦參屬植物，包括常用中藥：苦參、山豆根、槐花，及民間用藥毛苦參為實驗材料，首先進行苦參屬植物抗氧化成分含量與活性之評估，其次則以離體方式探討毛苦參與槐花對 H2O2誘導 SH-SY5Y 細胞氧化損傷之神經保護作用及其作用機轉。首先，在抗氧化成分及活性分析之研究結果發現，毛苦參、苦參、山豆根，和槐花均含總酚類、類黃酮類、生物鹼類及三萜類等抗氧化成分，且均具有抗氧化及清除自由基之作用，其中以槐花之抗氧化活性及自由基清除能力最佳，毛苦參次之。而在抗脂質過氧化及抗溶血活性上，槐花亦優於毛苦參。其次，在 H2O2 誘導 SH-SY5Y 細胞氧化損傷之研究結果發現，槐花及毛苦參可提升 H2O2 處理下 SH-SY5Y 細胞之存活率，且可清除胞外之 H2O2、降低胞內因 H2O2 引起之氧化壓力上升現象，另亦可減緩 H2O2 導致之粒腺體功能失調現象。至於槲皮素，亦可提昇 H2O2 處理下 SH-SY5Y 細胞之存活率。綜合上述結果，苦參屬植物毛苦參、苦參、山豆根和槐花均具有抗氧化活性，以槐花最佳，毛苦參次之。且槐花及毛苦參具神經細胞保護作用，其作用機轉可能與其所含總酚類成分及類黃酮含量之抗氧化活性，減少細胞內氧化壓力並清除細胞外自由基，以降低細胞損傷，減少粒腺體功能失調，而增加細胞之存活率有關。; Neurodegenerative diseases such as Alzheimer’s disease are closely related to free radical overloading and intracellular oxidative stress. Therefore, it is suggested that antioxidants may be useful for the prevention and treatment of neurodegenerative diseases. In this study the antioxidant constituents, and antioxidant activities of Sophora plants, S. flavescens root, S. tonkinensis root, S. japonica flower, and the herb’s stem and leaves of S. tomentosa were evaluated. The neuroprotective effects of S. tomemtosa and S. japonica on hydrogen peroxide induced oxidative damage in SH-SY5Y cells were examined in vitro. First, the experimental results demonstrated that S. tomentosa and S. japonica have enriched antioxidant components including total phenol and flavonoid among four Sophora plants and better in free radical scavenging and antioxidant activity. Furthermore, S. japonica has the better effects than S. tomentosa in inhibiting lipid peroxidation and hemolysis. Second, S. japonica and S. tomentosa elevate cell viability, decrease intracellular oxidative stress, scavenge extracellular hydrogen peroxide, and slow down the mitochondria dysfunction inducing by hydrogen peroxide. Quercetin can also advance the cell viability treated with hydrogen peroxide. In conclusion, Sophora plants including S. tomentosa, S. flavescens, S. tonkinensis and S. japonica possess antioxidative activities. S. japonica is superlative, and S. tomentosa is the second-best. S. japonica and S. tomentosa are both provided with neuroprotective effects resulting from its phenolic and flavonoid antioxidant components. They can enhance cell viability via decreasing intracellular oxidative stress, scavenging extracellular free radical and slowing down the mitochondria dysfunction in H2O2-treated SH-SY5Y cells.
顯示於類別:	[中國藥學研究所(已停用)] 博碩士論文

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