不同固定度之降解性神經管對再生神經功能恢復影響之評估; Evaluation of Genipin Crosslinked Gelatin Nerve Conduits With Different Fixation Rates on Nerve Function Recovery

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Title:	不同固定度之降解性神經管對再生神經功能恢復影響之評估;Evaluation of Genipin Crosslinked Gelatin Nerve Conduits With Different Fixation Rates on Nerve Function Recovery
Authors:	向士偉;hsiang shih-wei
Contributors:	中國醫藥大學中國醫學研究所
Keywords:	神經再生;明膠;綠梔子素;交聯;Nerve regeneration;genipin;gelatin;cross-liked;fixation index
Date:	2004
Issue Date:	2010-01-20
Abstract:	周邊神經斷傷在最近二十年來，已研究出許多可行的治療方式，最終目的皆希望可使神經的再生與修復更為完美，其中神經導管接合術常常使用於神經再生，而降解性的神經導管可提供避免二次手術的缺點，而明膠是最常使用製造的原料，但因為易遭受生物體的吸收而無法達到生醫材料的功用，因此由中藥梔子中的綠梔子素來增加其氨基的交聯度與固定度，本研究主要探討，以週邊神經修復為出發，探討不同交聯度之生物可吸收性之降解材料對於神經生長功能的影響，並使用運動學方面的評估，比較各組的差異性。我們以明膠為基材、綠梔子素為交聯劑製造出長度15 mm、重量0.05 + 10% g、內徑1.96 mm、固定指數分別為23±7％、36±2％、51±7％之降解性神經導管。SD大鼠皮下包埋一週、四週、八週之生物相容性評估顯示八週時有二次之異物發炎反應發生；三組的材料降解度則未達顯著差異。神經導管接合術適用性評估顯示51±7％的神經導管在SD大鼠體內，神經再生情形優於其他兩組，引導10 mm間距的神經再生成功率達100%、產生旺盛血管新生作用；但有神經再生生長遲滯及再生神經成熟度偏低的現象，在運動評估方面，與綠梔子交聯四十八小時的神經導管（51±7％），在坐骨功能指數（SFI）、感覺功能評估上發展比浸泡一小時（23±7％）之明膠管好，而在攀爬的實驗中發現。36±2％組與51±7％組的大鼠比23±7％組的大鼠需花較多的時間。; In the present study, we used a biodegradable genipin-crosslinked gelatin conduit (GGC) to bridge a 10 mm gap in the sciatic nerve of the Sprague-Dawley rat. We constructed the GGCs with different cross-linking degrees at 23±7%, 36±2%, and 51±7%, respectively. Their biological performance, such as the biocompatibility and the effectiveness as a guidance channel were evaluated by correlating morphometric and functional data of the regenerated nerve. As a result, we found that the regenerated nerves in the GGCs with 36% cross-linking degree had the most mature structure. In addition, GGCs with a higher cross-linking degree, such as those at 36% and 51%, had a better electro-physiological recovery. Therefore, we concluded that the GGCs with 36% cross-linking degree could provide the most appropriate growth environment for the regenerating nerves.
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