台灣金線連有效分劃對小鼠非特異性免疫的調節; Anoectochilus formosanus effective fraction modulated innate immunity in mice

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题名:	台灣金線連有效分劃對小鼠非特異性免疫的調節;Anoectochilus formosanus effective fraction modulated innate immunity in mice
作者:	徐致芬;Hsu, Chih-Fen
贡献者:	中國醫藥大學醫學研究所
关键词:	金線連;非特異性免疫;巨噬細胞;一氧化氮;Anoectochilus formosanus;innate immunity;macrophage;Nitric oxide
日期:	1993
上传时间:	2009-12-24 10:58:04 (UTC+8)
摘要:	台灣金線連(Anoectochilus formosanus HAYATA), 為傳統的民間藥草，在台灣常被使用在保肝的用途上。本研究，針對金線連有效分劃 (Anoectochilus formosanus effective fraction, AFEF)在非特異性免疫調節的功能評估，其中包括吞噬活力測定、NK細胞毒殺能力試驗，脾臟及淋巴結細胞次族群分型，細胞增殖能力，細胞激素分泌，及血清抗體分析等。BALB/c小鼠經餵食二星期及四星期後，脾臟淋巴細胞，腸繫淋巴細胞及腹腔中的巨噬細胞，實驗的結果顯示，在試驗動物體內口服體內試驗中，經分離單細胞來進行非特異性免疫調節的功能評估。巨噬細胞分別在二週及四週的給藥期間，其吞噬能力增加；而NK細胞則在餵食二週的AFEF (1500 mg/kg) 的毒殺能力增加，且活化態(CD69) T輔助型淋巴細胞的細胞次族群有顯著性增加；經ConA刺激48小時的腸繫淋巴細胞，所釋出IFN-??於AFEF (1500 mg/kg) 餵食二週下顯著性增加。爲了進一步探討巨噬細胞之吞噬活性的機轉，我們利用AFEF以體外培養液的模式刺激RAW 264.7細胞的吞噬活力，結果顯示，在AFEF 16 小時的刺激下細胞的吞噬能力、亞硝酸鹽 (Nitrite) 的釋出量、IL-2及IFN-??分泌量都有顯著性增加，綜合上述結果可以推測，AFEF能增強小鼠的非特異性免疫。; Anoectochilus formosanus effective fraction modulated innate immunity in mice Chih-Fen Hsu Institute of Medical Science,China Medical University Anoectochilus formosanus HAYATA (AF), a traditional pharmaceutical herb, had been used in hepatic protection in Taiwan. In this study, we will use the effective fraction of AF (AFEF) to modulate innate immunity including nautral killer (NK) cell cytotoxic assay, macrophage phagocytosis, splenocyte and lymph node subpopulation differentiate, cell proliferation, cytokine secreting assay and serum immunoglobulin assay. Various doses of AFEF administered to BALB/c mice for 2 and 4 weeks. Primary peritoneal macrophage harvest from mice that administered with various concentration of AFEF was direct to activate phagocytosis. On the other hand, AFEF can significant increase cytolysis effect in NK cell (1500 mg/kg) and that might be involved the activation of T helper cell in activating (CD69). AFEF can also significant increased IFN-??production from lymph node cells by ConA stimulation for 48 hours in administration AFEF (1500mg/kg). Further study to understand the mechanism of phagocytosis, in vitro AFEF-treated RAW 264.7 cells were analyzed in this study. AFEF can significant increases RAW 264.7 cell phagocytosis (100, 200 ?g/ml), by increased nitrite, IL-2 and IFN-? secretion. Taking all these data together, AFEF might be the active fraction in activation of innate immunity.
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