| 摘要: | 九節木Psychotria rubra (Lour.) Poir.為茜草科(Rubiaceae)之台灣常見植物；其民間用於治療扭傷、瘀傷、袪風除濕、風濕痛、痔瘡、消腫解毒、治感冒發熱。另且有報導從九節木乙醇提取物中分離出一種新的??化合物九節素(psychorubrin)對體外人鼻咽癌(KB)細胞呈現顯著的細胞毒性。 在本研究進行九節木藥理活性篩選及DPPH自由基清除能力之試驗，發現九節木莖之乙酸乙酯層抽出物，對腺癌細胞(SCM-1)及肝癌細胞(59T)兩種癌細胞皆有抑制作用；在DPPH自由基清除能力之試驗中，發現九節木之甲醇萃取物、氯仿、乙酸乙酯層及正丁醇層具有顯著的抗氧化活性。 本研究已從九節木之乾燥莖之甲醇粗抽物經分離純化及結構鑑定後，一共分離出 phytosterol (H-1), hexacosaconoic acid (H-2), ?-sitosterol-3-O-glucoside (H-3), lupeol (H-4), oleanolic acid (C-1), triacontanoic acid (C-2), vanillic acid (EA-1), EA-2等成分，其中EA-2應為??類化合物，??類化合物文獻記載具抗腫瘤及抗細胞毒性活性，oleanolic acid具有抗發炎活性、抗氧化的活性，vanillic acid在文獻記載也有抗氧化的活性，所以活性引導分離的實驗方法相當可行。; Psychortia rubra (Lour.) Poir. is a native plant of Taiwan belonging to the Rubiaceae family. It is a folk medicine being used for sprain, bruises, rheumatism, inflammation, hemorrhoids, traumatic injury, swelling, colds and fever. That a new naphthoquinone structure (psychorubrin) isolated from ethanolic extract of plant exhibited significantly cytotoxcity against KB cell line. In the assay for DPPH scavenging effect, except the water layer, all fractions showed good free radical scavenging effect. Our preliminary tests also showed that the extract of Psychortia rubra (Lour.) Poir. exhibited cytotoxic activity against SCM-1 and 59T cell lines. From the methanolic extract of this plant, seven chemical constituents were isolated and identified as phytosterol, hexacosaconoic acid, ?-sitosterol-3-O-glucoside, lupeol, oleanolic acid, triacontanoic acid and vanillic acid. Howere, the structure of EA-2 is still under investigation and is very likely to be a naphthoquinone. The structures of all the components were determined by spectroscopic method. Since this plant is rather common in Taiwan, further study and investigation of its active constituents may prove its potential biological activities and medicinal values. |
