美容醫學的市場近年來可說是發展迅速，潛力無限，尤其針對肝斑等色素沉著問題的解決，以往都得使用較具副作用的對苯二酚來治療，因此，針對中藥及各種天然物的萃取物之抑制黑色素形成及去斑美白的研究，更是目前發展的趨勢。本研究的目的是希望了解槐花萃取物的美白功效，研究中分別以95%乙醇、50%乙醇及純水來進行熱迴流萃取，並以不同溶劑萃取出之樣品的ㄧ半再加酸水解，再針對這六種不同的槐花萃取物樣品及市售常用之美白保養品成分(維他命C、BHA及熊果素)檢測其體外抑制酪氨酸酵素活性的能力，並以其中抑制率最高之實驗組(以純水萃取並加酸水解)樣品進行對人體纖維母細胞(WS1)及黑色素癌細胞(RPMI7951)培養之生長抑制影響的測定及對黑色素癌細胞培養時，針對細胞內液之酪氨酸酵素活性抑制的檢測。結果顯示以純水萃取並加酸水解之槐花萃取物具有最高的體外抑制酪氨酸酵素活性的能力，而對於WS1細胞培養之生長並無影響，但對黑色素癌細胞培養具有明顯之劑量相關性的生長抑制能力，但並無明顯對此細胞之細胞內液的酪氨酸酵素活性抑制能力。本實驗結論可推知槐花萃取物應為一有效且安全的美白成分，但其詳細的機轉則值得進ㄧ步的研究。; The marketing of medical cosmetics, especially for treating pigmented problems and mainly melasma is rapidly developing and has an unlimited potential in the current trend. Hydroquinone used to be used for bleaching with number of side effects been reported. Therefore, the studies on the Chinese herbs or other nature products for inhibiting melanogenesis are the tendency of researching this time. The purpose of this study is to assay the bleaching efficiencies of the extract of Sophora japonica. In our studies, distilled water, 50% alcohol and 95% alcohol were used as solvents to extract the dried Sophora flower buds. One half of each extract samples were hydrolyzed with sulfuric acid. The tyrosinase inhibiting efficiencies were evaluated by comparison the efficacies between extracted samples and commercial available bleaching agents (i.e. ascorbic acid, BHA and arbutin). The sophora extract by distilled water with further hydrolysis was found to be the most effective one based on its influences on the WS1 and RPMI 7951 cell culture studies. The results revealed that sophora extract didn’t disturb the cell growth in WS1 cell culture lines, but could inhibit cell growth with a dose-related effect in RPMI7951 cell culture lines. Herein, it was concluded that the Sophora japonica extracted with distilled water with further hydrolysis is a safe and an effective bleaching agent. However, the detailed mechanism should be merit to investigate.
