由電針刺激大白鼠俞穴釋出調控神經化學物質之探討; Manipulation of Neuro-chemicals by Electro-Acupuncture Stimulation in Rats

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Title:	由電針刺激大白鼠俞穴釋出調控神經化學物質之探討;Manipulation of Neuro-chemicals by Electro-Acupuncture Stimulation in Rats
Authors:	曾家順;Tseng, Chia-Shun
Contributors:	中國醫藥學院中西醫結合研究所
Keywords:	微透析技術;電針;葡萄糖;足三里穴;micro-dialysis;electro-acupuncture;glucose;zusanli;adenosine triphosphate
Date:	1992
Issue Date:	2009-12-14 11:30:12 (UTC+8)
Abstract:	中文摘要本項研究主要目的在於探討電針（Electro-acupuncture）施予大白鼠（Sprague Dawley Rat）之穴位對血糖之影響。分別以2 Hz頻率，固定強度（10 mA）來刺激大白鼠藉以瞭解電針針刺兩側足三里穴 Zusanli﹙ST 36 points﹚對血糖﹙blood glucose﹚、丙酮酸﹙Pyruvate﹚、乳酸﹙lactate﹚之影響和俞穴調控神經化學物質釋放之間相關性的變化。接著，比較血糖﹙blood glucose﹚、丙酮酸﹙Pyruvate﹚、乳酸﹙lactate﹚ 三種在以2 Hz或100 Hz不同頻率刺激昰否産生不同之影響，同時，比較大白鼠動物模型的穴區與非穴區以瞭解穴位的相關性。在調節血糖以及代謝機轉方面，利用全自動血液檢體收集儀分析電針前後對血糖(blood glucose), 丙酮酸(Pyruvate), 乳酸(lactate)之影響和調控神經化學物質釋放之間的差異，藉以瞭解電針對血糖(blood sugar), 丙酮酸(Pyruvate), 乳酸(lactate)之代謝及對細胞分泌的影響. 研究結果顯示，以低頻率﹙2 Hz﹚的電針有較佳的降糖效果，且有穴位的相關性，Glucose、Lacate 於插針﹙Baseline﹚後雖有些微上升但未呈顯著意義﹙Friedman Test, p>0.05﹚，L/G之Ratio值亦未有顯著的差異，經刺激電擊足三里30分鐘，初測Glucose、Lacate 雖有上升，L/G值有下降，然均未呈統計顯著之差異性，而後 Glucose、Lacate 均有下降，僅 Glucose 達顯著性差異﹙Wilcoxon Signed Ranks Test, p<0.05﹚，對於正常的大白鼠動物模型對血糖下降有良好效果，也與電針後可能經由增加了正常大白鼠之胰島素血中濃度使血糖有良好下降改變的效果，而Lacate與 L/G值均無顯著差異，終止電擊後約1小時30分，Glucose 則有顯著上升，而 Lacate與L/G值的變化均未達顯著差異。如今使用全自動血液檢體收集儀技術自體內移出化學物質而不用抽取體液且可導入化學化質以觀察反應。由全自動血液檢體收集儀技術取得的樣品不僅乾淨且可以立即使用各種不同分析技術加以分析，因此全自動血液檢體收集儀對於生物醫學和臨床研究上是非常有潛力的一種生物分析採樣技術，本研究應用電針在微透析連線技術刺激大白鼠俞穴釋出調控神經化學物質之分析提供日後新的研究方法(methodology); Abstract : BACKGROUND : Electrical stimulation of meridian points in the rat inhibits the nociceptive tail withdrawal reflex. It’s pain mechanisms are well documented, but the changes of extracellular glucose and lactate metabolites at cellular level are still unclear. The aim of the study was to evaluate these changes using a rat model in combination with the microdialysis technique for the analysis of extracellular neurochemicals. METHODS: Electrical stimulation was applied in anaesthetized intact rats (n =7) with 2 ms square pulses, 2Hz at the Zusanli points. A second electrical stimulation (2-Hz pulses) was delivered in 2 of them in the same spot. A second electrical stimulation of a different pulse (100-Hz pulses) was delivered. in another 1 in the same spot. A second electrical stimulation of 2-Hz pulses was delivered in another 3 rats in non-acpoints to compare the difference due to the different locations of stimulation. The stimulation lasted for 30 min each time it was delivered. A 90-min recovery period was allowed between the first electrical stimulation and the later treatment to avoid interruption. An automated micro-blood sample collector was used for examining the glucose, pyruvate and lactate value. CONCLUSIONS: electro-acupunctural signal has an influence on the biologic process of energy metabolism by mediating dynamic extracellular neuro-chemical changes. EA on limb acupoints of lower limbs could induce obvious decrease in glucose, increases in lactate metabolites and decrease in lactate/glucose ratio. Moreover, the high lactate/glucose ratio suggests that the cell have an increased anaerobic glucose metabolism.
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