1.釘地蜈蚣抗過敏、抗發炎及抗細胞增殖活性成分之研究 由七指蕨科 (Helminthostachyaceae) 植物釘地蜈蚣甲醇粗抽物之正己烷、氯仿分劃中，單離出一個混合物及四個化合物：ß-sitosterol & stigmasterol之混合物 (I)、hexadecanoic acid (II)、tetracosanoic acid (III)、ugonin H (IV) 及ugonin I (V)。Ugonin H (IV) 及ugonin I (V) 為首次從自然界分離得到的新化合物。 經抗細胞增殖試驗結果發現ugonin H (IV) 及ugonin I (V)有明顯抑制U937血癌細胞增殖活性。 而各化合物之抗過敏與抗發炎試驗仍在進行中。 2. 引藻細胞分化誘導及抗細胞增殖之活性成分研究 酯分劃中，單離出一個化合物及一個混合物：bis-(2-ethylhexyl) phthalate (CPA-1) 及hexadecanoic acid和octadecanoic acid之混合物 (CPA-2)。以上化合物均為已知，但都是第一次由引藻中分離得到。 經細胞分化和抗細胞增殖試驗發現bis-(2-ethylhexyl) phthalate (CPA-1) 具有抑制U937血癌細胞增殖之活性 (IC50＝58.4 μg/ml)。; 1. Studies on Antiallergic, Antiinflammatory and Antiproliferation Constituents of Helminthostachys zeylanica (L) Hook. Four compounds and one mixure ――ß-sitosterol and stigmasterol (I), hexadecanoic acid (II), tetracosanoic acid (III), ugonin H (IV) and ugonin I (V), have been isolated from the n-hexane and chloroform fractions of the methanolic extract of Helminthostachys zeylanica (L) Hook. Among the isolated compounds, ugonin H (IV) and ugonin I (V) are isolated for the first time. The results of the biological activities showed ugonin H (IV) and ugonin I (V) exhibited antiproliferation activities on U937 cells. However, other compounds are still under test. 2. Studies on Differentiation-induced and Antiproliferation Constituents of Cryptomonadales One compound and one mixture――bis-(2-ethylhexyl) phthalate (CPA-1), mixture of hexadecanoic acid and octadecanoic acid (CPA-2) have been isolated from the n-hexane, chloroform and ethyl aceteate fractions of the ethyl acetic extract of Cryptomonadales. These compounds were isolated for the first time from Cryptomonadales. The results of the biological activity investigation showed bis-(2-ethylhexyl) phthalate (CPA-1) exhibited antiproliferation activities on U937 cells (IC50＝58.4 μg/ml).
