中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/24188
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 29490/55136 (53%)
造訪人次 : 1552178      線上人數 : 382
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於CMUR ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/24188


    題名: 以AP-1為標的開發抗肝細胞轉形藥物;Development of the Anti-hepatocellular Transformation Compound by Using Activator Protein 1 as the Target
    作者: 吳泰賢;Wu Tai Hsien
    貢獻者: 中國醫藥學院醫學研究所
    關鍵詞: 吳茱萸;肝細胞轉形;AP-1;hepatocellular
    日期: 1991
    上傳時間: 2009-12-03 10:00:10 (UTC+8)
    摘要: Activator protein 1 (AP-1)為一種轉錄因子，可調控細胞的分裂、分化及轉形，而阻斷AP-1的活性也可抑制細胞的轉形，因此本研究主要就以AP-1為標的開發抗肝細胞轉形藥物。實驗是先利用經12-O-tetradecanoylphorbol-13-acetate (TPA)處理的Chang/AP-1及HepG2/AP-1重組細胞為材料，偵測98種中藥對細胞AP-1活性的影響。再從中篩選出抑制AP-1效果最好的10種中藥，分別是麻黃、吳茱萸、厚朴、大黃、檳榔、防己、知母、皂莢、苦參、甘草。進一步測試這10種中藥在抑制AP-1活性、細胞毒殺效應及肝細胞轉形的能力，其中對吳茱萸的甲醇萃取物、酸性乙醇及乙醇萃取物效果最佳，而且這些效應呈現劑量反應。因此由本研究的結果顯示，藉由中藥對AP-1活性的影響，可快速篩選出具有抗肝細胞轉形潛能的中藥，而本研究所篩選出的吳茱萸，在體外試驗中皆呈現明顯的抗肝細胞轉形效應，因此值得進一步利用動物試驗驗證其治療肝癌的效果。; Activator protein 1(AP-1), a nuclear transcription factor, plays important roles in cell proliferation, differentiation, and transformation. Previous study indicated that blocking of AP-1 activity is able to inhibit the cellular transformation. We therefore develop the anti-human hepatocellular compounds using AP-1 on the target in this study. Effects of 98 herbs on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced AP-1 activity in Chang/AP-1 and HepG2/AP-1 recombinant cells were investigated. Among the herbs, 10 herbs, which showed inhibition abilities on AP-1 activity, were selected. These herbs include Ephedra sinica, Evodia rutaecarpa, Magnolia officinallis, Rheum officinale, Arcea catechu, Stephanica tetrandra, Anemarrhena asphodeloids, Gledititsia sinensis, Sophora flavescens, and Glycyrrhiza uralensis. These herbs were further analyzed for their anti-AP-1 activities and anti-hepatocellular transformation activities by luciferase assay and anchorage-independent transformation assay. Results showed that Evodia rutaecarpa exhibited the significant inhibition ability on AP-1 activity and hepatocellular transformation in a dose-dependent manner. Moreover, the methanol, acid alcoholic, and alcoholic extracts of E. rutaecarpa were responsible for their anti-hepatocellular transformation abilities.
    顯示於類別:[醫學研究所] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    534007.pdf336KbAdobe PDF1114檢視/開啟


    在CMUR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

     

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋