本研究應用溴化氫與環氧乙烷反應生成2-溴乙醇之原理，希望結合固相微萃取技術，發展新的空氣中環氧乙烷採樣分析方法。 文獻記載擴散式被動採樣的原理可適用於固相微萃取之纖維探針，而溴化氫與環氧乙烷反應生成2-溴乙醇之原理，亦可有效地應用於主動式與被動式空氣採樣，因此本研究以固相微萃取的纖維探針為採樣器之主體，再以頂空法將溴化氫裹覆於纖維上作為吸附劑。研究中首先測試不同纖維、尋求適合溴化氫裹覆的條件，然後決定裹覆與熱脫附所需之適當時間，再利用空氣採樣袋或動態標準氣體製造系統，進行擴散式被動採樣，樣本收集完畢後，直接插入氣相層析質譜儀之注射部進行樣本之脫附與分析。 由測試多種不同之纖維發現，以Carboxen/Polidimetilsilossano (Car/PDMS)纖維最能有效裹覆溴化氫，經30秒的頂空裹附，在理論採樣率為2.7×10-2㎝3/min的假設下，溴化氫的量約可提供45小時連續在1ppm (TLV-TWA) 的環氧乙烷濃度下採樣所需。熱脫附時間的探討發現，脫附 5分鐘時，脫附效果達99%。而在環境中風速為0至50pfm、相對溼度10至80﹪的情況下，採樣器的採樣率為(2.98±0.12)×10-2 cm3/min(R2=0.9816)。方法偵測極限為1.1×10-4μg(環氧乙烷)。採樣器的壽命和樣本儲存穩定性至少有2天，樣本回收率分別為95﹪及107﹪。 利用固相微萃取技術，本研究的採樣器構造簡單、攜帶方便、並可重複使用，且具有將取樣、萃取、濃縮及導入分析物等合併於單一步驟、不需使用溶劑、減少萃取時間、及提高分析處理速度等優點。本研究的結果顯示採樣器可以有效地採集環氧乙烷，未來將繼續驗證溫度及其他等環境條件對採樣器的影響，並與其他現有的方法進行平行比對。; The purpose of this study is to develop a new passive sampler for ethylene oxide (EtO) in air based on the technique of solid phase microextraction (SPME) and the use of hydrobromic acid (HBr). The passive sampler was constructed by the fiber assembly of SPME. Different fibers were first tested by head space extraction with HBr solutions to determine the optimized condition for HBr coating. The desorption condition was also determined. The validation of the sampler was performed by using gas bag technique and/or by using the standard gas generation system. After sampling, the sampler was inserted into the injection port of gas chromatography/mass spectrometry (GC-MS) for thermal desorption and sample analysis. Carboxen/Polidimetilsilossano (Car/PDMS) fiber was found to adsorb HBr with best performance among the fibers tested. The amount of HBr coated after 30 sec head space extraction can be used for 44 hours sampling at 1 ppm (TLV-TWA) of EtO when the sampling constant is2.7×10-2㎝3/min (theoretical). The desorption efficiency was 99% when the time for thermal desorption was 5 min. The experimental sampling constant was (2.98±0.12)×10-2 cm3/min(R2=0.9816) determined by wind velocity in 0~50pfm and 10~80﹪relative humidity. Method Detection Limits is 1.1×10-4μg(EtO). Samples collected and stored for a minimum of 2 days resulted in average recoveries that ranged from 95% to 107%. The sampler designed in this study has the advantages of solvent-free, short extraction time and high reproducibility. Also the method combines sampling, extraction, concentration, and sample introduction into one step. The results showed that the ethylene oxide can be collected effectively. However, more experiments are needed to validate the performance of the sampler on the effects of temperatures, relative humidities, face velocities as well as the collection efficiencies of ketones.
