中藥白芨對皮膚傷口癒合之研究; Effect of Bletilla striata on cutaneous wound healing

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题名:	中藥白芨對皮膚傷口癒合之研究;Effect of Bletilla striata on cutaneous wound healing
作者:	陳怡廷;Chen Yi Ting
贡献者:	中國醫藥學院中國醫學研究所
关键词:	白芨;皮膚傷口癒合;明膠;綠梔子素;Bletilla striata;cutaneous wound healing;gelatin;genipin
日期:	1991
上传时间:	2009-12-03 09:34:51 (UTC+8)
摘要:	皮膚損傷常見於各種外傷或疾病，例如挫傷、燒傷、燙傷、糖尿病、褥瘡、傷口久不癒合等。一個暴露的傷口不僅造成疼痛，也成為外來微生物入侵的管道。在面積較大而且深及真皮層的傷口中，因傷口癒合較慢，導致癒合後出現瘢痕攣縮，甚至影響功能及美觀。在傳統中醫藥白芨常用於止血、胃、十二指腸潰瘍、皮膚燒燙傷，具有止血、去腐生肌的功效。所以在本實驗中選擇白芨為主要藥物，探討白芨對深層皮膚損傷癒合的作用。本實驗分為三部分，第一部份實驗探討白芨的基本生化作用，在致突變性方面採用Ames test分析，結果顯示白芨並不具致突變性；在細胞毒性測試可觀察到，低濃度的白芨水溶液可刺激角質細胞增生；以蛋白質電泳分析法分析白芨所含蛋白質含量，顯示其蛋白質含量低於0.005%，具低過敏性。第二部份實驗以不同濃度的白芨水溶液，對天竺鼠背部深層皮膚損傷癒合之影響。結果顯示：在生理食鹽水、10%白芨水溶液、20%白芨水溶液三組中，於術後三天，10%白芨水溶液及20 %白芨水溶液，能明顯促進肉芽組織生長；在術後第五天、七天組中10%白芨水溶液及20%白芨水溶液與控制組相比，有明顯促進角質細胞移行、增加血管新生、肉芽組織生長的能力，尤其以20%白芨水溶液組更加明顯。因白芨具有促進傷口癒合功效，所以第三部份實驗以不同分子量的明膠以綠梔子素交聯來包覆白芨，製作新型傷口敷料，延長白芨在傷口處的藥物釋放，實驗共分成四組，分別為分子量300、225、75、及對照組，結果發現相較於分子量75及對照組，分子量225、300二組能提供促進的傷口癒合的能力。關鍵字：白芨、皮膚傷口癒合、明膠、綠梔子素; Patients with full-thickness burns or after excision of large areas of skin usually suffer from a sub-stantial loss of dermal tissue. In general, these wounds are repaired with split-skin grafts. Cultured human epithelial autografts (CEA) are alternatives for wound closure. Injured tissue also can be repaired by covering with a non-medicated or medicated wound dressing. In addition wound dressing can be used for skin lesion such as, burn injury, surgical wounds and chronic ulcer. The Chinese herb Bletilla striata has been used to treatment peptic ulcer and cutaneous wound in clinics. In the present study, we first evaluated the mutagenic activity of Bletilla striata. As a resutt, Bletilla striata did not have mutagenic activities in the Ames test using Samonella typhimurium strains TA97, TA98, TA100, and TA102, with and without metabolic activation. Electrophretic analysis of protein on SDS-page was used to detect protein content and protein contain below to 0.005% in extract solution of Bletilla striata. Cytotoxicity test was arranged to assess the Bletilla striata cytotoxicity to human keratinocytes. Results of MTT assay showed the cell counts increased with low concentration of Bletilla striata solution. In vivo tests, we evaluated the effects of Bletilla striata gel (10% , 20%) on repairing the dermal damages of guinea pigs. Results showed Bletilla striata gel (10% , 20%) could promote keratinocytes migration, angiogenesis, and granulation tissue formation. In order to control the drug efficiency, we used a genipin-fixed gelatin to encapuslate the with Bletilla striata gel. Different bloom numbers of gelatin (300, 225, 75) were used to prepare wound dressings with different degradation rates. Three types of wound dressings were then applied on the full-thickness dorsal skin defect of Sprague-Dawley rat. At postoperative day 10 and 20, histological results showed an enhancement of wound healing in all the genipin-fixed gelatin matrices, especially the one with bloom number 300.
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