肝細胞癌蛋白活化酵素C與血管內皮生長因子基因表現的關聯性及臨床意義; Clinical Significance of and Relationship Between Protein　Kinase C and Vascular Endothelial Growth Factor Gene Expression in Hepatocellular Carcinoma

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題名:	肝細胞癌蛋白活化酵素C與血管內皮生長因子基因表現的關聯性及臨床意義;Clinical Significance of and Relationship Between Protein　Kinase C and Vascular Endothelial Growth Factor Gene Expression in Hepatocellular Carcinoma
作者:	林忠義;Lin C.Y.
貢獻者:	中國醫藥學院醫學研究所
關鍵詞:	蛋白活化酵素C;血管內皮生長因子;PKC;VEGF
日期:	1990
上傳時間:	2009-12-03 09:27:23 (UTC+8)
摘要:	為要評估蛋白活化酵素Ｃ(protein kinase C, PKC)及血管內皮生長因子(vascular endothelial growth factor, VEGF)在肝癌中的基因表現所扮演的角色及兩者間的關係，15位在本院受手術切除治療的肝癌病人，列入研究。　　使用反轉聚合酵素連鎖反應(reverse transcriptase polymerase chain reaction, RT-PCR)及免疫組織化學染色(immunohisto chemistry, IHC)對腫瘤及非腫瘤部位進行偵測。　　PKC與VEGF基因表現之相關性與患者之一般資料，臨床表徵，實驗室及病理檢查結果作一系列的分析。　　腫瘤血管顯影程度與VEGF的免疫化學染色蛋白質表現有關聯性(P=0.0003) ，而與腫瘤大小、病理分級、胎兒蛋白高低、門脈侵犯、包膜有無浸潤無關聯性，腫瘤與鄰近的肝細胞皆有VEGF免疫染色，VEGF與PKCβ1皆在腫瘤血管內皮細胞中呈現免疫染色，而肝癌細胞VEGF mRNA與PKCβ1 mRNA在RT-PCR的表現無關聯性 (P=0.68)，腫瘤與非腫瘤部位的VEGF mRNA或PKCβ1 mRNA在RT-PCR中表現無關聯性。　　總之，VEGF在肝癌腫瘤血管增生中有其重要地位，而PKCβ1，也在VEGF引導的腫瘤血管增生扮演一定的角色。; 　　To evaluate the relationship between protein kinase C (PKC) and vascular endothelial growth factor (VEGF) gene expression in hepatocellular carcinoma, 15 patients who had undergone curative hepatic resection for HCC in the past one year were enrolled into this study, VEGF and PKC gene expression were evaluated for HCC and the liver by semiquantitave reverse-transcription polymerase chain reaction (RT-PCK) and immunostaining. The results of these assays were compared with the patient’s general data, clinical manifestations and laboratory data and pathological features. 　　Tumoral stain on angiography was significantly correlated with VEGF expression in HCC by immunostaing (P=0.0003), however, tumor size, histological grade of malignancy, the level of α-feto protein, portal vein invasion, and the microscopic infiltration of cancer cells into the tumor capsule were not correlated with VEGF expresion in HCC. VEGF was detected in tumor and surrounding liver tissues. VEGF and PKCβ1 were also found in tumoral microvessels by immunostaing. The OD values of VEGF mRNA and PKCβ1 mKNA, mHCC were not correlated by RT-PCR assay. The OD values of VEGF mRNA and PKCβ1 mKNA were not correlated be tween tumor and non-tumor portions. 　　In conclusion, VEGF play an important role in Angiogenesis of HCC, PKCβ1 lies on the pathway by which VEGF mediated this reaction. A larger series of studies are necessary to elucidate its clinic implication in HCC.
顯示於類別:	[醫學研究所] 博碩士論文

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