醫療廢棄物焚化爐勞工之CYP1A1及GSTM1基因多性型與DNA-protein crosslinks 的相關性; Influence of genotypic polymorphism of CYP1A1 and GSTM1 on DNA-protein crosslinks in incinerator workers in hospital

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Title:	醫療廢棄物焚化爐勞工之CYP1A1及GSTM1基因多性型與DNA-protein crosslinks 的相關性;Influence of genotypic polymorphism of CYP1A1 and GSTM1 on DNA-protein crosslinks in incinerator workers in hospital
Authors:	蔡慶慧;ChingHuei Tsai
Contributors:	中國醫藥學院環境醫學研究所
Keywords:	醫療廢棄物焚化爐;CYP1A1;GSTM1;DPC;Medical waste incinerator;DPC;CYP1A1;GSTM1
Date:	1990
Issue Date:	2009-12-03
Abstract:	摘要本研究要探討國內醫療廢棄物焚化爐作業勞工之易感性代謝多型性基因CYPlA1 、GSTM1 與DNA-protein crosslinks（DPC）間的相關性。研究對象來自全省各大醫院醫療廢棄物焚化爐作業勞工，共31名，另外依照焚化爐作業勞工之性別、年齡、抽煙習慣等變項選取配對31名對照組，並使用問卷調查其內容包含有性別、年齡、抽煙、喝酒、工作年資及每日處理垃圾量等。由結果顯示，焚化爐作業勞工與對照組的基本特性，不論在年齡、性別、抽煙、吃檳榔及喝酒，二組間的比較沒有統計上的差異。易感性代謝多型性基因之分佈頻率，在焚化爐作業勞工具有CYP1A1同型合子野生型與異型合子野生型之頻率為48.4%，同型合子變異型為51.6%，而對照組則各為41.9%及58.1%，二組間沒有統計上的差異。GSTM1之比率在勞工組非無效型之頻率為58.1%，無效型者為41.9%，而對照組則各為74.2%及25.8%。以DPC而論，焚化爐勞工明顯高於對照組，其值分別為1.5%及0.9%，以t-test檢定分析達到統計上顯著差異(p<0.01)。CYP1A1與GSTM1基因型與DPC值間的比較分析，發現無論是勞工組或對照組，其基因型與DPC值間均無統計上的差異。在DPC的多變項迴歸分析，只有有無暴露之變項，顯示有統計上的差異，其他變項均無差異。關鍵字: 醫療廢棄物焚化爐， CYP1A1，GSTM1 ，DPC; Abstract The issue of environmental pollution in incinerating the medical waste has been concerned by the general public. In this study, DPC in peripheral blood lymphocytes were measured as an endpoint of cytogenetic damage. However, this kind of study is frequently complicated by variants confounding factors, both endogens (sex, age) and exogenous(life style, smoking , alcohol abuse ,diet habits etc.)In this study ,31 medical waste incinerator workers and 31 normal subjects as a matched control were enrolled. The purpose of the present study is to investigate whether genetic polymorphisms in CYP1A1 and GSTM1 could explain some of the variation in DPC. DPC levels were significantly higher in the medical waste incinerator workers compare to the control group (1.5 versus 0.9 DPC% , p<0.01).We measured the association between GSTM1 null genotype, which results in loss of enzyme activity, and DPC levels, hypothesizing that subjects with this genotype would have higher levels of DPC. However, there was no association with the GSTM1 polymorphism and DPC levels both in workers (for GSTM1 non null , DPC%=1.5; for GSTM1 null, DPC%=1.5; p=0.4)and controls (for GSTM1 non null , DPC%=0.9; for GSTM1 null, DPC%=1.1; p=0.27).Similarly, there was no association with the CYP1A1 MspⅠpolymorphism and DPC levels in workers(for CYP1A1 MspⅠwt/wt+wt/vt , DPC%=1.6; for CYP1A1 MspⅠvt/vt , DPC%=1.5; p=0.3) and controls (for CYP1A1 MspⅠwt/wt+wt/vt , DPC%=0.9; for CYP1A1 MspⅠvt/vt , DPC%=1.0; p=0.17). Higher DPC levels in medical waste incinerator workers suggest that this group are at increased risk for exposing to ambient air pollution contaminated with PAHs . Key word: medical waste incinerator, DPC (DNA protein crosslinks), CYP1A1, GSTM1.
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