摘 要 含氧自由基( Reactive Oxygen Species ; ROS )目前已被研究出它會使人體內DNA 造成氧化性的損傷，進而造成基因的突變形 成癌症。而8-Hydroxy-2''-deoxyguanosine（8-OHdG）是體內含氧自由基( ROS )造成DNA損傷後的生物指標，會造成G : C to T : A的複製錯誤。本研究目的在比較乳癌患者與正常婦女尿液中8-OHdG含量的差異，且希望藉由問卷來了解8-OHdG與其他危險因子在乳癌患者與正常婦女之關係。並進一步希望能以8-OHdG當 作乳癌患者中DNA氧化傷害的生物指標。 結果顯示在喝咖啡方面，在健康婦女有喝咖啡尿液中8-OHdG濃度比無喝者略高，但並未達統計上差異；但在惡性乳癌腫瘤患者中喝咖啡者與否卻有統計上差異（p<0.05）。在惡性乳癌腫瘤患者(81.81nM)尿液中8-OHdG濃度為顯著性較健康婦女(33.14 nM)為高，且有統計上之差異（p<0.001）；尿液中8-OHdG濃度經肌肝酸及體重校正後其結果亦是相同；若用ROC（Receiver Operational Characteristics）決定切點時，尿液中8-OHdG濃度值定在45nM時則敏感度為69﹪與特定度為75﹪。惡性腫瘤患者尿液中8-OHdG濃度也較良性腫瘤患者為高，但未達統計上差異（p=0.052），當8-OHdG切點為36nM時則敏感度為73﹪與特定度為80﹪。在對惡性乳癌腫瘤患者分期後尿液中8-OHdG濃度比較方面，本研究結果上顯示不論在經肌肝酸或體重校正後，第一期惡性腫瘤患者尿液中8-OHdG濃度高於第二期與第三期患者，有依次遞減之趨勢，不過由於個案數較少尚未達統計上顯著的差異。因此由本研究可利用尿中8-OHdG濃度可表示乳癌所產生之DNA損害性，對人體早期危害提供良好的生物指標。; Abstract Oxygen radicals have been shown to cause extensive damage to DNA, which it leads to gene mutations. Urinary 8-hydroxy- 2’-deoxyguanosine（8-OHdG）has been widely studied as a biomarker for detection oxidative stress as it is one of the most specific forms of DNA damaged by reactive oxygen species. 8-OHdG is thought to be promutagenic lesion since this lesion induces G：C to T：A transversion unless repaired prior to replication. The objective of this study is to compare the urinary 8-OHdG levels between breast cancer patients and healthy women. Our results indicated coffee drinking did not significantly correlate with 8-OHdG in healthy women, but it correlated with urinary 8-OHdG levels in breast cancer patients. There were significant higher urinary 8-OHdG levels in breast cancer patients（81.81nM）compared to healthy women (33.14nM). Urinary 8-OHdG levels after creatinine and body weight corrected were compared between breast cancer patients and control women the results were the similar. When the point of urinary 8-OHdG levels was cut at 45nM based the receiver operational characteristics, the sensitivity and specificity for diagnosis of breast cancer were 69﹪and 75%, respectively. When the point of 8-OHdG levels was cut at 36nM for classification between the benign and malignant breast tumor, the sensitivity and specificity for diagnosis of breast cancer were 73﹪and 80%, respectively. Urinary 8-OHdG levels in stage I of cancer was higher than that of stageⅡ and stageⅢ of cancer, however, it was not significantly. Our concluded urinary 8-OHdG is used to as a biomarker of oxidative stress in breast cancer.
