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| 題名: | 臺灣蒲公英組織培養之研究;Tissue Culture of Taraxacum formosanum |
| 作者: | 羅國瑞;Lo, Kuo~Jui |
| 貢獻者: | 中國醫藥學院中國藥學研究所 |
| 關鍵詞: | 蒱公英;Taraxacum formosanum |
| 日期: | 1990 |
| 上傳時間: | 2009-12-02 11:52:40 (UTC+8) |
| 摘要: | 臺灣蒲公英(Taraxacum formosanum KITAGAWA)依中外生藥學文獻均指為菊科(Compositae)、蒲公英屬(Taraxacum)之植物。我國文獻，蒲公英最早記載於唐•新修本草，列於草部下品之下，名為蒲公草，自古即為清熱解毒，治療婦人乳癰腫之傳統要藥。蒲公英一名最早見於宋•圖經本草，惟以蒲公英為正名，則始於明代本草綱目。近年來，臺灣因土地的開發、農藥的濫用加以外來種蒲公英的競爭，使特有種臺灣蒲公英已不多見，只零星分佈於大甲以北沿岸沙地，為避免臺灣特有種蒲公英成為瀕臨絕種之植物，利用組織培養大量繁殖或以細胞培養生產二次代謝物為本研究之目的。茲將結果摘錄如下: 1.種子接種於含有0.5 mg/l BA、3% sucrose和1% Difco agar之1/2 MS 培養基中，經一週後可長成幼苗。 2.將臺灣蒲公英種子培養於含1.0 mg/l NAA、0.5 mg/l BA之1/2 MS基本鹽類培養基中，可誘導癒合組織產生。 3.1/2 MS基本鹽類添加 0.5 mg/l NAA、1.0 mg/l kinetin、3% sucrose及1.0% Difco agar，pH值5.7±0.1之固體培養基，於暗培養及25±1℃之恆溫下，是臺灣蒲公英癒合組織生長之最佳條件。 4.添加(10% v/v)的椰子汁或馬鈴薯泥皆不利於臺灣蒲公英癒合組織之生長。 5.添加( 500, 1000, 2000 mg/l)peptone或水解酪蛋白皆不利於臺灣蒲公英癒合組織之生長。 6.1/2 MS基本鹽類添加 0.5 mg/l BA、3% sucrose及1.0% Difco agar，pH值5.7±0.1之固體培養基，於2000 lux光照及25±1℃之恆溫下，可誘導臺灣蒲公英葉片長出不定芽。 7.自葉片誘導之不定芽，於2000 lux光照及25±1℃之恆溫下，培養於含有1/4 MS~1/2 MS無機鹽、0.5mg/l NAA、及3~5% sucrose之培養基，可長出具有良好根系之植株。 8.將組織培養苗移植於混合不同比利之介質中，2週後調查存活率，結果發現以vermiculite：peat moss＝1：1之介質存活率較佳。; Summary Taraxacum spp (Compositae) were first recorded in Sin-Sho-Pen-Tsao of the Tang Dynasty under inferior category.Taraxacum formosanum is one of the trantional Chinese medicinal herbs used in the treatment of matitis, tonsillits, cholestasis and cleaning the waste of the liver. Due to limited geographical distribution and indiscriminate collection, T. formosanum is now rarely found in its natural habitat. Over development of land for many use and introduction of alien species of T. officinale also resulted in reduction population of T. formosanum. The purpose of this study was to establish the tissue culture techniques for mass propagation of Taraxacum formosanum. The results of the present investigation are summarized as: 1.Nearly 100% germination rate was obtained, when seeds of T. formosanum were cultured on a medium containing half-strength MS basic salts supplemented with 0.5mg/l BA, 3% sucrose and 1.0% Difco agar. 2.A medium containing half-strength MS salts, 1.0 mg/l NAA, 0.5 mg/l BA, 3% sucrose and 1% Difco agar can induce callus from T. formosanum seed. 3.A medium containing half-strength MS salts, 0.5 mg/l NAA, 1.0 mg/l kinetin, 3% sucrose and 1.0% Difco agar (pH=5.7) was the most suitable medium for inducing callus formation of T. formosanum. The cultures were maintained at 25±1℃ under dark. 4.Addition of 10%(v/v) coconut milk (CM) or potato homogenate is not good for callus growth of T. formosanum. 5.Adding (500,1000,2000 mg/l) peptone or casein hydrolysate(CH) is not good for callus growth of T. formosanum. 6.Adventitious buds can be induced from leaves when cultured on a medium containing half-strength MS basic salts, 0.5mg/l BA, 3% sucrose and 1% Difco agar (pH=5.7). The cultures were maintained at 25±1℃ under a photoperiod of 14-h light (2000 lux fluorecent light) and 10-h dark cycle. 7.For rooting of adventitious buds, which are induced on leaves, buds were cultured on a medium containing 1/4~1/2 MS basic salts, 0.5mg/l NAA, 3~5% sucrose and 1% Difco agar (pH=5.7). The cultures were maintained at 25±1℃ under a photoperiod of 14-h light (2000 lux fluorecent light) and 10-h dark cycle. 8.The plantlets of T. formosanum produced from tissus culture could be transplanted into a mixture of vermiculite and peat moss (1:1), and grown successfully under outdoor conditions. |
| 顯示於類別: | [中國藥學研究所(已停用)] 博碩士論文
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