延胡索對於缺血性-再灌流大鼠腦梗塞效用之研究 研究生 廖恩賜 中國醫藥學院 中國醫學研究所 中文摘要 根據傳統中醫的理論腦中風屬於血瘀證的一種﹐所以常用活血化瘀的中藥或方劑來治療﹐根據中醫本草典籍的記載延胡索具有破血利氣的作用﹐因此本研究的目的是利用動物模式來探討延胡索對於腦梗塞型中風的效用和它的機轉。方法是將重量300-450克的雄性Sprague-Dawley（SD）大鼠的兩側頸總動脈和右中大腦動脈的血流阻斷90分鐘﹐經24小時再灌流後觀察它們的神經缺損﹐然後將它們犧牲取腦放入鼠腦塑膠模型中﹐並切成2 mm的腦切片、選取前額葉的六片經2% 2,3,5-triphenyltetrazolium（TTC）染色後﹐經由電腦圖像處理分析系統中﹐計算梗塞面積、結果是以梗塞面積和全面積的比率表示。於腦血流阻斷前的30分鐘分別腹腔注射延胡索100 mg/kg和50 mg/kg﹐並與對照組做比較。結果顯示延胡索100 mg/kg和50 mg/kg能減小缺血－再灌流損傷大鼠的腦梗塞面積﹐以及延胡索100 mg/kg能減小缺血性腦梗塞大鼠的神經缺損。另外﹐延胡索不會影響肝和腎功能﹐但它會增加周邊血液的紅血球數目(RBC)和血球容積比(Hematocrite)。 我們的結論是延胡所可以減少缺血－再灌流損傷大鼠腦梗塞的面積和神經缺損﹐因此我們認為延胡索可以用來治療人類的梗塞型腦中風﹐特別是在急性期﹐至於它的機轉須再做進一步的探討。 關鍵辭：腦中風 ，腦梗塞，梗塞面積，延胡索; Effect of Rhizoma Corydalis on Cerebral Infarct in Ischemia-reperfusion Injuried Rats EN-SZU LIAO Institute of Chinese Medical Sciences,China Medical College Abstract According to the theory of Traditional Chinese Medicine，cerebral infarction results from blood stasis，and the method of quickening the blood and dispelling stasis is used to treat cerebral infarct.Rhizoma Corydalis(RC) is a Chinese herb which is considered to have a action of quickening the blood and dispelling stasis.The aim of the presnt was to investigate the effect of RC on cerebral infarct in ischemia-reperfusion injuried rats.The model of focal cerebral infarct was developed by occluding the bilateral common carotid arteries and the right middle cerebral artery for 90 min. After 24 hrs reperfusion,evaluated the neurological status and then the rats were killed and the brain tissue was stained with 2,3,5-triphenyl-tetrazolium chloride(TTC).The areas of cerebral infarct were calculated,and the change in the area of cerebral infarct were used as an index to evaluate the of RC on cerebral infarct.The results indicated that pretreatment with intraperitoneal injection of RC 50mg/kg and 100mg/kg reduce the area of cerebral infarct and 100mg/kg reduce the neurological defecit.Furthermore, RC won't effect the liver and renal function and increase red blood cell and heamatocrit . Our conclusion is that RC can reduce cerebral infarct area and neurological defecit of ischemia-reperfusion injuried rats,suggesting it may be useful in treatment of cerebral infarct in humam,especially in acute stage and the possible mechainisms are worth both laboratory and clinical study. Key Words:Stroke,Cerebral infarction,Infarct size,Rhizoma corydalis
