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請使用永久網址來引用或連結此文件:
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| 題名: | 天麻活性成分香莢蘭醇對kainic acid誘發大白鼠癲癇發做效用之研究
The effects of vanillyl alcohol on rats with kainic acid-induced epileptic seizures |
| 作者: | 李佳容;Chia-Jung Lee |
| 貢獻者: | 中國醫藥學院中國醫學研究所 |
| 關鍵詞: | 癲癇;香莢蘭醇;kainic acid;wet dog shakes;自由基;SOD;Epilepsy;vanillyl alcohol;kainic acid;wet dog shakes;free radicals;SOD |
| 日期: | 1999 |
| 上傳時間: | 2009-11-26 16:08:35 (UTC+8) |
| 摘要: | 癲癇是一種慢性疾病，目前雖大部分的癲癇發作可以藥物甚至外科治療得到控制，但藥物的副作用及需長期服用仍是患者的一大困擾。尋求有效而且更安全的治療是大家致力追求的目標，天麻為傳統中醫用以治療癲癇的常用藥之一，為進一步了解其抗癲癇作用與明確機轉，我們選用天麻的有效成份香莢蘭醇(vanillyl alcohol)作為研究治療用藥。本研究以Sprague-Dawley (SD)大白鼠腹腔注射kainic acid ( KA，12mg/kg) 誘發癲癇發作並觀察它的時間經過，同時分別事先投予Vanillyl alcohol 100mg/kg， 200mg/kg，觀察Vanillyl alcohol的抗癲癇效果。另外將30隻老鼠分為五組:除一組僅給PBS外，餘皆在KA注射前30分鐘分別給與Vanillyl alcohol 100mg/kg, 200mg/kg與PBS 1ml/kg, 觀察腦波及wet dog shakes (WDS)發作。一小時後將這些老鼠犧牲，取血測量Luminol-CL 和Lucigenin-CL的數目，同時取腦，將腦分為左右大腦與小腦三部份，分別測量其SOD(superoxide dismutase)的活性。結果顯示WDS於KA注射後60分鐘它的發作頻率達到高峰。Vanillyl alcohol 100mg/kg， 200mg/kg均可減少KA所誘發WDS的次數，且可降低血液中luminol-CL counts與lucigenin-CL counts，及降低大腦中的SOD活性，但對小腦的SOD則無變化。 我們的結論是Vanillyl alcohol 有抗痙攣作用，同時有自由基的清除作用。由於大腦中SOD的活性降低，但對小腦則無變化，因此我們猜測Vanillyl alcohol的抗癲癇作用主要來自於它對自由基的清除作用，或抑制自由基的生成，而非促進SOD的活性。; Epilepsy is a common chronic disease that could be congenital or induced by many factors such as toxin, metabolic imbalance or brain injury. Almost all of the modern anticonvulsants have the side effects of drowsy, dizziness, headache or paralysis. Chinese traditional medicines may offer another choice for epileptic patients. Gastrodia elata Bl. (GE) is a traditional Chinese herb that is usually used to treat convulsive disorder. The aim of this study was to investigate the anticonvulsant effect of vanillyl alcohol (VA) which is the active component of Gastrodia elata Bl., and the physiological mechanisms of its action in rats. A total of 55 male Sprague-Dawley (SD) rats were used for study. Twenty five of these rats received intraperitoneal injection (i.p.) of VA 100mg/kg, VA 200mg/kg or no treatment 30min prior to kainic acid (KA, 12mg/kg) administration, respectively. Behavior and EEG were monitored from 15min prior to drug administration to 3 hours after KA administration. The remaining 30 rats were divided into 5 groups of 6 rats as follows: 1) Normal group: no treatment was given; 2) Control group: received KA (12 mg/kg) i.p.; 3) VA 100 group and 4) VA 200 group; received VA 100 mg/kg, VA 200 mg/kg i.p. 30 min prior to KA administration, respectively. 5) Contrast group, received PBS i.p. 30 min prior to KA administration. Throughout the experimental course, behavioral observation, EEG and EMG recordings were performed until 1 hour after KA administration. All 30 rats were used to measure the Lucigenin-CL counts and Luminol-CL counts in the whole blood, and superoxide dismutase (SOD) activities in the brain. Our results indicated that VA 100 mg/kg and VA 200 mg/kg may decreased the incidence of KA-induced wet dog shakes in rats. They also decreased the KA-induced free radicals. VA-treated animals showed lower activity of SOD in cortex of both hemispheres than control group, even than normal group. But no similar response was seen in the cerebellum. These findings suggest that the anticonvulsant effect of VA possibly result from its suppressive effect of free radicals formation, not from the induction of SOD activity. |
| 顯示於類別: | [中國醫學研究所] 博碩士論文
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