This invention discloses a new and effective method for obtaininglarge quantity of osthole with high purity from herbal medicines, moreparticularly, from Cnidii fructus. In comparison with the conventionalcolumu chromatographic method,it has the advantages of rapid, less timeconsuming, and no environmental pollution. In the present invention ithas also been proved experimentally that Cnidii fructus, including ostholeand extract of Cnidii fructus, has significant inhibition and lethal effectson Trichomonas vaginalis. Therefore, an anti-Trichomonas vaginalispreparation containing osthole as the main ingredient with essentialexcipient (s) can be developed.