Purpose: Increasing studies have suggested that free radical-initiated peroxidation of biomembrane is associated with a variety of pathological events, including cardiovascular diseases, some cancers, and neurodegenerative diseases. The 2,2-azobis(2-amidinopropane hydrochloride (AAPH)-induced hemolysis has been studied extensively as a model of oxidative damage of biomembranes. Sun-Huang-Xie-Xin-Tang (SHXXT) is common fomula of Chinese herbs, including Rhei Rhizoma, Scutellariae Radix and Coptidis Rhizoma. This study attempted to investigate the effects of SHXXT and its polyphenol constituents on the hemolysis induced by AAPH. Methods: Based on our previous studies on the metabolic pharmacokinetic of polyphenols, the conjugated metabolites of polyphenols were prepared by using rats. Rat erythrocyte suspension was incubated with AAPH and polyphenol metabolites at 37℃ for 7 hr. The absorbance at 540 nm was measured to calculate the inhibition on hemolysis.For in vivo study, two groups of rats were administered with SHXXT decoction and equivalent volume of water in parallel design. Blood samples were withdrawn at specific time points. After removal of buffy coat, the erythrocyte and plasma were added AAPH and incubated at 37℃ for 7 hr. The absorbance at 540 nm was measured. The percentages of water of inhibition on hemolysis were compared between two groups by using unpaired Student t-test. Results: The in vitro studies indicated that emodin conjugated metabolites exhibited anti-hemolysis effect. The blood withdrawn from rats fed SHXXT decoction also exhibited hemolysis resistant effect. Conclusions: Administration of SHXXT decoction to rats has protected erythrocyte membrane against oxidation. The antioxidation activity of SHXXT can be accounted for by the presence of polyphenols .
關聯:
2nd World congress of the board of Pharmaceutical sciences of FIP
