Effects of CLC-107 on proliferation and apoptosis in U-937 leukemia cells?

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 藥學院 > 藥物化學研究所 > 會議論文 > Item 310903500/22766

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/22766

题名:	Effects of CLC-107 on proliferation and apoptosis in U-937 leukemia cells?
作者:	(Shu-Fang Wang);(Li-Chen Chao);(Chein-Ting Chen);(Shi-Hong Zhuang);(Jai-Sing Yang);郭盛助(Sheng-Chu Kuo);黃麗嬌(Li-Jiau Huang)?
贡献者:	藥學院藥物化學研究所;?
日期:	2005-09-4?
上传时间:	2009-09-07 12:19:38 (UTC+8)
摘要:	CLC-107, a novel furopyrazole compound, was examined for cells proliferation inhibition effect on human or mouse cancer cell lines include leukemia cells (HL-60, U-937, RAW 264.7 and WEHI-3), lung cancer carcinoma (CH27, A-549 and H1355), hepatoblastoma (Hep G2 and Hep 3B) and colon adenocarcinoma (COLO 205). We used propidium iodide (PI) incorporation and MTT assay to determine the proliferation rate on CLC-107 treatment. Our data showed after 48 h of treatment with CLC-107, concentration- and time-dependent decrease in cell proliferation rate of U-937 and WEHI-3 cells. CLC-107 treated U-937 and WEHI-3 cells presented typical characteristics of apoptosis as further evidenced by TUNEL/DAPI double-staining and internucleosomal DNA fragmentation by electrophoresis. CLC-107 obviously induced loss of mitochondrial membrane potential in U-937 cells treated for 24 h, and apparent release of cytosolic cytochrome c, down-regulation of Bcl-2 and up-regulation of Bax protein by Western blot analysis. CLC-107 induced apoptosis was mainly mediated by activation of caspase-3 by caspases activity assay. These results indicated that the ability of CLC-107 to inhibit cell proliferation might be inducted apoptosis on U-937 leukemia cells.?
關聯:	2005藥物化學研討會?
显示于类别:	[藥物化學研究所] 會議論文

文件中的档案:

没有与此文件相关的档案.

在CMUR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....