| 摘要: | Osteoblast Proliferation and Maturation by Kinsenoside from Anoectochilus formosanus Hui-Ya Ho(何惠雅)a, Chia-Chi Lo(羅佳祺)b, Susumu Kitanaka(北中進)d, Jin-Bin Wu(吳金濱)a,* aGraduate Institute of Pharmaceutical Chemistry, China Medical University , 91 Hsueh Shih Road, Taichung,Taiwan bJen-Li Biotech Co. Ltd., No.47, Yongfong Rd., Taiping City, Taichung County 411, Taiwan (R.O.C.) dCollege of Pharmacy, Nihon University, Chiba 274-8555, Japan E-mail address: [email protected] (J.B. Wu). (何惠雅) Anoectochilus formosanus is an orchidaceous perennial herb distributed only in Taiwan and Okinawa (Japan) and has been used as a valued folk remedy for fever, pain, lung, liver diseases, and in underdeveloped children. Recently, this herbal drug was also used to cure hypertension, diabetes mellitus, consumption and nephritis, as well as osteopenia in ovariectomized rats. In this study, we investigated Kinsenoside that is an aliphatic glycoside (3-(R)-3-b-D-glucopyranosyloxybutanolide) isolated from A. formosanus. in vitro effect of proliferation and alkaline phosphatase (ALP) activity in MG-63 cultured human osteoblasts. We found that significantly increased proliferation and ALP activity. This effect was markedly reduced by PD 98059, an inhibitor of the extracellular regulated kinase (ERK) pathway, and by cycloheximide, inhibits protein synthesis. The results suggest that Kinsenoside can stimulate osteoblastic activity and may represent new pharmacological tools for the treatment of osteoporosis. Shih, C.C., Wu, Y.W., Lin, W.C., J. Ethnopharmacol , 2001 , 77, 233–238. Aiko Ito, Ryoji Kasai, Kazuo Yamasaki, et al., phytochemistry, 1993, 33, 1133-1137 |
