KPP95 is a lytic bacteriophage isolated from hospital wastewater using Klebsiella pneumoniae 10693 as the indicator cell. High titers of the phage, approximately 3 ? 1012 plaque forming units, can be obtained by propagation in shaking cultures. It has an icosahedral head with tail, which is similar to T-even phage, as revealed by electron microscopy. The genome size of KPP95, calculated by summing up the lengths of the restriction fragments, was estimated to be 175 kb. The genome can only be digested by the endonucleases AseI and SspI, whose recognition sequences contain either A or T only, suggesting that the DNA contain unusual bases. In SDS-polyacrylamide gel electrophoresis of the phage particles, more than 25 proteins including one major coat protein of 46 kDa were visualized. Interestingly, KPP95 can also infect some ESBL strains, including Enterobacter cloacae and Serratia marcescens, but not Escherichia coli.
