Abstract. Arylamine carcinogens need to be activated by N-acetyltransferase(NAT) to form active metabolites that lead to induce cancer. Berberine has been reported to induce cell death (apoptosis) and to inhibit NAT activity in human cancer cell lines (Bladder, colon and brain tumor cells) Therefore, our object was to investigate the effect of berberine on growth, NAT activity and gene expression in bacteria strain Salmonella typhi. The growth inhibition of S. typhi was determined by measuring absorbance by an optical density (OD at 650nm) using spectrophotometer. The NAT activity was determined by high performance liquid chromatography for measuring the levels of 2-aminoflorene and N-acetyl-2-aminofluorene. The result demonstrated that a 24 hour berberine treatment decrease the % of bacteria growth and levels of N-acetyl-2-aminofluorene in S. typhi. Western blotting and flow cytometry were used for examining the levels of NAT and it indicated that berberine decreased the levels of NAT in S. typhi. Polymerase chain reaction (PCR) was used for examining the gene expression of NAT (mRNAT NAT) and it was indicated that berberine affect mRNA NAT expression in S. typhi.
關聯:
The 7th International Conference of Anticancer Research
