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請使用永久網址來引用或連結此文件:
http://ir.cmu.edu.tw/ir/handle/310903500/2058
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| 題名: | CTGF enhances migration and MMP-13 up-regulation via alphavbeta3 integrin, FAK, ERK, and NF-kB-dependent pathway in human chondrosarcoma cells |
| 作者: | 譚思濰(Tan Tzu-Wei);賴志河(Chih-Ho Lai);黃俊寅(Chun-Yin Huang);楊維宏(Wei-Hung Yang);陳賢德(Hsien-Te Chen);許弘昌(Horng-Chaung Hsu);馮逸卿(Yi-Chin Fong);湯智昕(Tang Chih-Hsin)* |
| 貢獻者: | 醫學院醫學系學士班藥理學科 |
| 日期: | 2009-05 |
| 上傳時間: | 2009-08-19 17:24:00 (UTC+8) |
| 摘要: | Tumor malignancy is associated with several features such as proliferation ability and frequency of metastasis. Connective tissue growth factor (CTGF), a secreted protein that binds to integrins, modulates the invasive behavior of certain human cancer cells. However, the effect of CTGF on migration activity in human chondrosarcoma cells is mostly unknown. Here we found that CTGF increased the migration and expression of matrix metalloproteinase (MMP)-13 in human chondrosarcoma cells (JJ012 cells). RGD peptide, alphavbeta3 monoclonal antibody (mAb) and MAPK kinase (MEK) inhibitors (PD98059 and U0126) but not RAD peptide inhibited the CTGF-induced increase of the migration and MMP-13 up-regulation of chondrosarcoma cells. CTGF stimulation increased the phosphorylation of focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK). In addition, treatment of JJ012 cells with NF-kappaB inhibitor (PDTC) or IkappaB protease inhibitor (TPCK) inhibited CTGF-induced cell migration and MMP-13 up-regulation. Stimulation of JJ012 cells with CTGF also induced IkappaB kinase alpha/beta (IKK alpha/beta) phosphorylation, IkappaBalpha phosphorylation, p65 Ser(536) phosphorylation, and kappaB-luciferase activity. The CTGF-mediated increases in kappaB-luciferase activities were inhibited by RGD, PD98059, U0126 or FAK, and ERK2 mutant. Taken together, our results indicated that CTGF enhances the migration of chondrosarcoma cells by increasing MMP-13 expression through the alphavbeta3 integrin, FAK, ERK, and NF-kappaB signal transduction pathway. 2009 Wiley-Liss, Inc. |
| 關聯: | JOURNAL OF CELLULAR BIOCHEMISTRY107(2):345~356 |
| 顯示於類別: | [醫學系] 期刊論文
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