中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/1985
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 29490/55136 (53%)
造訪人次 : 1507655      線上人數 : 759
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於CMUR ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/1985


    題名: Analysis of upregulated cellular genes in pseudorabies virus infection: use of mRNA differential display.
    作者: 項千芸(Chien-Yun Hsiang);侯庭鏞(Tin-Yun Ho);(Lin, C.H.);(Wu, K.);(Chang, T.J.)*
    貢獻者: 醫學院醫學系學士班微生物學科
    關鍵詞: Pseudorabies virus;mRNA differential display;Positive selection
    日期: 1996-10
    上傳時間: 2009-08-19 17:23:29 (UTC+8)
    摘要: Virus infection usually alters the host cell and shuts off the synthesis of cellular macromolecules. In order to screen the upregulated cellular transcripts during pseudorabies virus (PRV) infection, we employed the mRNA differential display technique. The screen is based on positive selection at the mRNA level for genes expressed in normal cells but increased in corresponding PRV-infected cells. Over 14000 species of mRNA, isolated from mock-infected and PRV-infected Madin-Darby bovine kidney cell at l h post infection, were screened, and 40 candidate clones were recovered. Southern blot analysis revealed that 17 out of 40 candidate clones, were enhanced in PRV-infected cells. Partial DNA sequences demonstrated that 17 clones were distinct cellular genes, including those encoding the modulators of signal transduction (saposin, 14-3-3, adenylate kinase, adenylyl cyclase, protein kinase C-α), those encoding the components of translation (fau, ribosomal proteins S11, L31, L36), other cellular genes (peptidase, cyclin E, rchl, oligo-C-rich single-stranded nucleic acid binding protein, rap, arginyl-tRNA synthetase), and two unknown genes. Thus, this study identifies successfully the transcriptionally regulated cellular genes which are associated with PRV infection. Furthermore, this study provides support for the use of mRNA differential display as a method to rapidly isolate differentially expressed genes in virus infection.
    關聯: JOURNAL OF VIROLOGICAL METHODS 62(1):11~19
    顯示於類別:[醫學系] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML324檢視/開啟


    在CMUR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

     

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋