Although aloe-emodin has been reported to be an antineoplastic agent for some of human cancer cell lines, affect leukocytes functions in vitro are not be demonstrated. We wanted to know whether aloe-emodin would affect the phagocytosis of macrophages, the activity of natural killers and the expression of cytokines in leukocytes from Sprague-Dawley rats. Therefore, leukocytes were collected from rats then placed into the culture plastes then the functions of macrophages and natural killers were evaluated and examined by flow cytometric analysis. Incubations of leukocytes with or without various concentrations of aloe-emodin causes a decrease of phagocytosis from macrophages and activity of natural killers. Both effects are dose-dependent. Cytokines were evaluated by ELISA and the results indicated that aloe-emodin decreased the levels of IL-1 IL-2, IL-12, TNF-α and TGF-β. Those results were also confirmed by PCR assay for mRNA expression of those examined cytokines.
關聯:
SEVENTH INTERNATIONAL CONFERENCE OF ANTICANCER RESEARCH
