Histidine residue at position 226 is critical for iodide uptake activity of human sodium/iodide symporter

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 醫學院 > 醫學系 > 期刊論文 > Item 310903500/1904

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題名:	Histidine residue at position 226 is critical for iodide uptake activity of human sodium/iodide symporter
作者:	吳世祿(Shih-Lu Wu);侯庭鏞(Tin-Yun Ho);梁基安(Ji-An Liang);項千芸(Chien-Yun Hsiang)*
貢獻者:	醫學院醫學系學士班生化學科
日期:	2008-11
上傳時間:	2009-08-19 17:22:58 (UTC+8)
摘要:	The sodium/iodide symporter (SLC5A5; also known as NIS), a transmembrane glycoprotein principally in the thyroid gland, is responsible for the accumulation of iodide necessary for thyroid hormones. Our previous study indicated that a novel exon 6 deletion (residues 233–280) in SLC5A5 loses the iodide uptake activity. Herein we characterized the role of His-226 in iodide transport of SLC5A5. His-226, a highly conserved extracellular residue among SLC5A5 homologs, was replaced with alanine, aspartic acid, glutamic acid, or lysine. All the SLC5A5 mutants were expressed normally in the cells and targeted correctly to the plasma membrane. However, all of the mutants displayed severe defects in iodide uptake, suggesting that His-226 was critical for iodide uptake. Kinetic analysis further showed that mutation at His-226 led to a dramatic decrease in Vmax. These findings suggested that the decreased levels of iodide uptake activity of SLC5A5 mutants resulted from lower catalytic rates. In conclusion, our data first identified the involvement of extracellular charged amino acid residue in the iodide uptake ability of SLC5A5.
關聯:	JOURNAL OF ENDOCRINOLOGY 199(2):213~219
顯示於類別:	[醫學系] 期刊論文

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