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http://ir.cmu.edu.tw/ir/handle/310903500/18636
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| 題名: | Study the function of C1qRp/CD93 |
| 作者: | 黃蕙君(Huang,Huey-Chun) |
| 貢獻者: | 健康照護學院醫學檢驗生物技術學系 |
| 日期: | 2005-03-26 |
| 上傳時間: | 2009-09-04 15:51:38 (UTC+8) |
| 摘要: | Study the function of C1qRp/CD93 J.-P. Tsai,H.-C. Huang, G.-Y. Shi, H.-L.Wu Department of Medical Laboratory Science and Biotechnology, National Cheng King University, Tainan, Taiwan Abstract： C1qRp/CD93 is a type I cell surface glycoprotein predominantly expressed on myeloid lineage cells, microglia cells, endothelial cells, and early stem cell precursors. Human C1qRp/CD93 consists of 631 amino acids and contains a signal peptide followed by a carbohydrate recognition domain (CRD), five EGF-like domains, a mucin-like region juxtaposed to the transmembrane region, and a short cytoplasmic tail. C1qRp/CD93 was originally named for its suggested participation in the enhancement of FcR and complement receptor (CR1)-mediated phagocytosis. The CD93-/- mice showed no growth abnormalities in their development, but had a significant phagocytic defect in the clearance of apoptotic cells in vivo. However, other reports suggested that C1qRp/CD93 was not required for enhancing phagocytosis in vitro. The function of C1qRp/CD93 remains controversial. On the other hand, recent reports have described the possibility that C1qRp/CD93 might mediate leukocyte–endothelial interactions.In this study, we cloned the gene of C1qRp/CD93 from human histiocytic lymphoma U937 cells and investigated the functions in A2058 cells. We found that the cell proliferation rate was significantly increased in C1qRp/CD93-expressed A2058 cells. The fluorescence of C1qRp/CD93 –expressed and C1qRp/CD93 delete lectin like –expressed A2058 cells distributed on membrane and microvilli whereas C1qRp/CD93 – delete cytoplasmic trail clones-expressed clones distributed in cytosol. Moreover, we used flow cytometry to explore the role of C1qRp/CD93 in PMA-stimulated THP-1 differentiation. Our preliminary results demonstrated that the C1qRp/CD93 expression was down-regulated by PMA from day 1 to day 5, and recovered to normal at day 7. These results suggested that the expression of C1qRp/CD93 might play a role i |
| 關聯: | 第20屆生物醫學聯合學術年會 |
| 顯示於類別: | [營養學系暨碩士班 ] 會議論文
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