中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/18577
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    题名: Involvement of lectin-like domain of thrombomodulin in cell adhesion
    作者: 黃蕙君(Huang,Huey-Chun)
    贡献者: 健康照護學院醫學檢驗生物技術學系
    日期: 2003-11-14
    上传时间: 2009-09-04 15:50:17 (UTC+8)
    摘要: Thrombomodulin (TM) is an integral membrane glycoprotein that is a potent anticoagulant factor. TM may also possess functions distinct from its anticoagulant activity. Presently, the influence of TM on cell adhesion was studied in TM-negative melanoma A2058 cells transfected with green fluorescent protein (GFP)-tagged TM (TMG) or lectin domain-deleted TM [TMG(DL)]. Confocal microscopy demonstrated that both TMG and TMG(DL) were distributed in the plasma membrane. TMG-expressed cells grew as closely clustered colonies, with TM localized prominently in the intercellular boundaries. TMG(DL)-expressed cells grew singly. Overexpression of TMG, but not TMG(DL), decreased monolayer permeability in vitro, and tumor growth in vivo. The cell-to-cell adhesion in TMG-expressed cells was Ca2+-dependent and was inhibited by monoclonal antibody against the lectin-like domain of TM. The effects of TM-mediated cell adhesion were abolished by addition of mannose, chondroitin sulfate A, or chondroitin sulfate C. In addition, anti-lectin-like domain antibody disrupted the close clustering of the endogenous TM-expressed keratinocyte HaCaT cell line derived from normal human epidermis. Double-labeling immunofluorescence staining revealed similar distributions of TM and actin filament in the cortex region of the TMG-expressed cells. Thus, TM can function as a Ca2+-dependent cell-to-cell adhesion molecule. Binding of specific carbohydrates to the lectin-like domain is essential for this specific function.
    關聯: 台灣生物化學及分子生物學學會秋令營
    显示于类别:[營養學系暨碩士班 ] 會議論文

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