Molecular Cloning and Expression of the Gene Encoding a Putative b-Galactosidase-Stabilizing Protein form Xanthomonas campestris pv. campestris

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Title:	Molecular Cloning and Expression of the Gene Encoding a Putative b-Galactosidase-Stabilizing Protein form Xanthomonas campestris pv. campestris
Authors:	楊翠青(Yang,Tsuey-Ching);(郭莉婷);(曾義雄)
Contributors:	健康照護學院醫學檢驗生物技術學系
Date:	2004-12-12
Issue Date:	2009-09-04 15:49:28 (UTC+8)
Abstract:	Other than the three annotated b-galactosidase genes, a novel b-galactosidase gene (galD) has been identified from Xanthomonas campestris pv. campestris. In this study, a 405-aa protein, ORF405, has been showed to markedly affect significant the b-galactosidase activity of GalD. Insertional inactivation of the orf405 gene decreased, although not completely abolished, the b-galactosidase activity of galD, while co-overexpression of galD and orf405 caused an apparent elevation on the b-galactosidase activity of GalD. This phenotype implied that ORF405 may be a b-galactosidase-stabilizing protein.
Relation:	第三十八次中華民國微生物學會
Appears in Collections:	[Graduate Institute of Nutrition ] Proceedings

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