Xanthomonas campestris 新b-半乳糖甘脢基因之特性描述

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Title:	Xanthomonas campestris 新b-半乳糖甘脢基因之特性描述
Authors:	楊翠青(Yang,Tsuey-Ching)
Contributors:	健康照護學院醫學檢驗生物技術學系
Date:	2003-12-07
Issue Date:	2009-09-04 15:49:23 (UTC+8)
Abstract:	With three annotated b-galactosidase genes, Xanthomonas campestris cannot grow well using lactose as the sole carbon source. Previously, a lactose-utilizing strain (Xc17L) with elevated b-galactosidase activity was isolated by mutagenesis with nitrous acid. The gene (galD) responsible for the enzyme elevation was studied by Tn5 transposition of Xc17L, cloning of the wild-type gene, nucleotide sequence analysis, genetic complementation, and Western blotting. The results indicated that galD (1740-bp long with 65.7% G+C content, encoding a protein of 579 aa with a MW of 66) is indeed a novel b-galactosidase gene. Using the GalD sequence for blast, a new family of b-galactosidases from other organisms has been found.?
Relation:	中華民國微生物學會第三十七次年會
Appears in Collections:	[Graduate Institute of Nutrition ] Proceedings

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