The genotoxic potential and chemopreventive effects of two commonly used anti-abortion herbal medicines on ENU-induced transplacental genotoxicity in mice {{Thirteen Her! bs An-Tai In{{ (THATI) and {{Bow-Tai-Tang{{ (BTT) are two commonly used anti-abortion herbal medicines. Due to their extensive use, it is of great interest regarding their genotoxic and anti-genotoxic potential to pregnant women and their offspring. In this study, the potential genotoxic and chemopreventive effects of THATI and BTT in pregnant ICR mice and their fetuses were examined by Micronuclei assay. For genotoxicity group, aqueous extracts of THATI and BTT were given by gavage from gestation day (GD) 12 to 17. For chemoprevention group, N-ethyl-N-nitrosourea (ENU) was given by intraperitoneal injection (ip) at a dose of 90mg/kg on GD 17 two hours after the treatment of THATI and BTT from GD 12 to 17. ! Blood samples were collected on GD 19 to examine the frequencies of mi cronuclei, a marker for chromosome damage, in maternal and fetal reticulocytes (RET). There is no statistically significant difference among the THATI and BTT experimental groups and the negative control group in maternal body weight gain and RET/normochromatic erythrocytes (NCE) ratio. However, RET/NCE ratios in fetuses were markedly higher than those in pregnant dams. Treatment of THATI at the dose of 17.6 g/kg and 5.9 g/kg and BTT at the dose of 9.8 g/kg did not induce a statistically significantly increase in frequencies of micronuclei in maternal and fetal reticulocytes. Moreover, pretreatment with BTT but not THATI resulted in statistically significant decrease in the frequencies of ENU-induced micronucleated reticulocytes, with an inhibition ratio of about 30% and 20%, in the maternal blood and fetal blood, respectively. These data suggests that BTT but not THATI possess the chemopreventive effect with no observable cytotoxicity in ICR mice and their fetuses.?
關聯:
The 36th Annual Meeting of the European Environmental Mutagen Soc
