Curcumin Induced Apoptosis of Human Colon Cancer Colo 205 Cells through the Production of ROS, Ca+2 and the Activation of Caspase-3

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Title:	Curcumin Induced Apoptosis of Human Colon Cancer Colo 205 Cells through the Production of ROS, Ca+2 and the Activation of Caspase-3
Authors:	陳光偉(Chen,Guang-Wei);蘇進成(Su,Chin-Cheng);林昭庚(Lin,Jaung-Geng);李德茂(Te-Mao Li);鍾景光(Chung, Jing-Gung)
Contributors:	中醫學院中醫學系學士班中醫外傷學科
Date:	2005-06-24
Issue Date:	2009-09-03 15:47:31 (UTC+8)
Abstract:	Summary Curcumin (diferuloylmethane), the yellow pigment in turmeric (Curcuma longa), is known to inhibit proliferation of cancer cells by arresting them at various phases of the cell cycle and is known to induce apoptosis in tumor cells. Curcumin-induced apoptosis mainly involves the activation of caspase-3 and mitochondria-mediated pathway in various cancer cells of different tissues of origin. In the present, we investigated the induction of apoptosis from colon cancer colo 205 cells to underscore the need for further understanding of the multiple mechanisms of cell death unleashed by curcumin. We examined cytotoxicity and apoptosis in curcumin treated colon cancer colo 205 cells by using flow cytometry and the results demonstrated that curcumin induced cytotoxicity and apoptosis in these examined cells and that these effects are dose-and time-depedent. We also used DAPI staining to confirm curcumin induced apoptosis. We also used flow cytometry to show curcumin induced reactive oxygen species (ROS) and Ca+2 productions and decreased the levels of mitochondria membrane potential and induced caspase-3 activity. We also used Comet assay to show curcumin induced DNA damage in these examined cells. We also used Western blotting to analyze the levels of Bax, Bcl-2, cytochrome C, p53, and p21 in curcumin treated cells and the results demonstrated that curcumin promoted the expression of Bax, cytochrome C, p53 and p21 but inhibited the expression of Bcl-2. These observations together suggest that curcumin may have a possible therapeutic potential in colon cancer patients.
Relation:	第二十屆天然藥物研討會
Appears in Collections:	[School of Chinese Medicine] Proceedings

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