Cardiac Fas-dependent and mitochondria-dependent apoptosis in ovariectomized rat

中國醫藥大學機構典藏 China Medical University Repository, Taiwan > 健康照護學院 > 物理治療學系(停用) > 期刊論文 > Item 310903500/1610

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题名:	Cardiac Fas-dependent and mitochondria-dependent apoptosis in ovariectomized rat
作者:	李信達(Shin-Da Lee);郭薇雯(Wei-Wen Kuo);(Ying-Jui Ho);(Ann-Chi Lin);蔡長海(Chang-Hai Tsai);(Hsueh Fang Wang);(Chia-Hua Kuo);(Ai-Lun Yang);黃志揚(Chih-Yang Huang);(Jin-Ming Hwang)*
贡献者:	健康照護學院物理治療學系
日期:	2008-11
上传时间:	2009-08-19 17:04:04 (UTC+8)
摘要:	Background Very limited information has been published regarding cardiac apoptosis in menopausal women or in those after bilateral oophorectomy. The purpose of this study was to evaluate whether cardiac Fas-dependent (type I) and mitochondria-dependent (type II) apoptotic pathways are activated in ovariectomized rats. Methods Thirty-two female Wistar rats at 6–7 months of age were randomly divided into sham-operated group (Sham) and bilateral ovariectomized group (OVX). Two months after the operation, the cardiac characteristics, myocardial architecture, and two major apoptotic pathways in the excised left ventricle from rats were measured by histopathological analysis, Western blotting and reverse transcription polymerase chain reaction (RT-PCR), and positive TUNEL assays. Results The whole heart weight, the left ventricular weight, the ratios of whole heart weight to tibia length, and the ratios of left ventricle to tibia length were significantly increased in OVX relative to Sham. Abnormal myocardial architecture, enlarged interstitial spaces, more minor cardiac fibrosis, and more cardiac TUNEL-positive apoptotic cells were observed in OVX. The key components of Fas-dependent apoptosis (TNF-alpha, Fas ligand, Fas death receptors, Fas-associated death domain (FADD), activated caspase 8, and activated caspase 3) and key components of mitochondria-dependent apoptosis (Bad, Bax, Bax-to-Bcl2 ratio, cytosolic cytochrome c, activated caspase 9, and activated caspase 3) were significantly increased in OVX hearts. Conclusions The absence of female ovaries will activate the cardiac Fas-dependent and mitochondria-dependent apoptotic pathways, which might provide one of possible mechanism for developing heart failure in post-menopause women.
關聯:	MATURITAS61(3):268~277
显示于类别:	[物理治療學系(停用)] 期刊論文

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