中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/1587
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cmu.edu.tw/ir/handle/310903500/1587


    题名: Diallyl disulfide inhibits N-acetyltransferase activity and gene expression in human esophagus epidermoid carcinoma CE 81T/VGH cells
    作者: 游富順;游春淑;(Chun-Shu Yu);林景彬(Jing-Pin Lin);(Ssu-Cheng Chen);(Wan-Wen Lai);鍾景光*
    贡献者: 健康照護學院口腔衛生學系
    日期: 2005-07
    上传时间: 2009-08-19 17:00:27 (UTC+8)
    摘要: Individuals can be classified into rapid or slow acetylators based on the N-acetyltransferase (NAT) activity which is believed to
    affect cancer risk that is related to environmental carcinogen exposure. Diallyl disulfide (DADS) is a naturally occurring organosulfur
    compound, from garlic (Allium sativum), which exerts anti-neoplasm activity. In this study, we investigated the inhibitory
    effects of DADS on NAT activity and gene expresseion (NAT mRNA) in human esophagus epidermoid carcinoma CE 81T/
    VGH cells. NAT activity was measured by the amounts of N-acetylation of 2-aminofluorene (AF) and non-acetylation of AF by
    high performance liquid chromatography on cells treated with or without DADS. The amounts of NAT enzymes were examined
    and analyzed by Western blot. NAT gene expression (NAT mRNA) was examined by polymerase chain reaction and cDNA microarray.
    DADS decreased the amount of N-acetylation of AF in human esophagus epidermoid carcinoma CE 81T/VGH cells in a
    dose-dependent manner. Western blot analysis indicated that DADS decreased the levels of NAT protein in CE 81T/VGH cells.
    PCR and cDNA microarray experiments showed that DADS affected NAT1 mRNA expression in CE 81T/VGH cells. DADS affect
    NAT activity due to the inhibition of gene expression (NAT1 mRNA) and the decreasing of the protein levels of NAT in CE 81T/
    VGH cells.
    關聯: FOOD AND CHEMICAL TOXICOLOGY 43(7):1029~1036
    显示于类别:[口腔衛生學系] 期刊論文

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