中國醫藥大學機構典藏 China Medical University Repository, Taiwan:Item 310903500/1580
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    题名: Combining the Capilia TB assay with smear morphology forthe identifi cation of Mycobacterium tuberculosis complex
    作者: 沈光漢;陳昭賢(Chao-Hsien Chen);(C-H. Hung);吳崑銘(K-M. Wu);(C-F. Lin);Y-W. Sun;陳建華(J-H. Chen)*
    贡献者: 健康照護學院二年制呼吸治療學系
    关键词: Mycobacterium tuberculosis complex;Capilia TB assay;BD ProbeTec ET (CTB) system;serpentine cord
    日期: 2009
    上传时间: 2009-08-19 16:59:42 (UTC+8)
    摘要: "SETTING: Many hospitals in Taiwan use the fully automated BACTEC Mycobacterial Growth Indicator Tube (MGIT) 960 system to identify mycobacteria in clinical specimens, while the labour-intensive BD ProbeTec ET (CTB) system or biochemical methods are used to identify Mycobacterium tuberculosis complex (MTC) in mycobacterially positive BACTEC cultures.

    OBJECTIVE: To evaluate whether the Capilia TB assay can be used to replace the BD ProbeTec ET (CTB) system or biochemical methods for identifying MTC in BACTEC cultures.

    DESIGN: Mycobacterially positive BACTEC cultures were collected and MTC in the cultures was identified using biochemical methods. MTC was identified using serpentine cording in smears, the Capilia TB assay or the BD ProbeTec ET (CTB) system, and the results were compared.

    RESULTS: Using 233 mycobacterially positive BACTEC cultures, the sensitivity and specificity of identification of the Capilia TB assay were respectively 96.9% and 98.6%, while those of the BD ProbeTec ET (CTB) system were respectively 99.4% and 97.3%. Combining the Capilia TB assay with serpentine cording in smears led to 100% specificity for intersected results and 100% sensitivity for combined results.

    CONCLUSION: The Capilia TB assay can be used to identify MTC in BACTEC cultures. By combining the assay with serpentine cording in smears, false-positives and -negatives may be reduced."
    關聯: INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE13(3):371~376
    显示于类别:[二年制呼吸治療學系] 期刊論文

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