人體尿液中營養調查方法之建立--尿液中黃酮類微營養素之分析

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Title:	人體尿液中營養調查方法之建立--尿液中黃酮類微營養素之分析
Other Titles:	Urine Analysis of Flavonoid Micronutrients
Authors:	李珮端(Pei-Dawn Lee Chao);徐素蘭(Hsiu,Su-Lan);侯鈺琪(Hou,Yu-Chi)
Contributors:	藥學院藥學系
Keywords:	微營養素;黃酮類;代謝物;尿液分析;flavonoids;micronutrients;pharmacokinetic;urine
Date:	2005-12-31
Issue Date:	2009-09-01 16:27:04 (UTC+8)
Abstract:	許多跨國流行病學研究顯示，日常黃酮類之攝食量與心血管疾病、癌症、神經退化等慢性疾病之罹患率息息相關，因此黃酮類被認為是一類微營養素。本計畫建立了精確簡便的HPLC/UV方法，分析尿液中之黃酮類微營養素。健康男性自願受試者飲用葡萄柚汁、柳橙汁、豆漿等本土飲料後，定量尿液中的 naringenin、hesperetin、genistein、daidzein、equol等黃酮之配醣體、非醣體或其結合態代謝物 sulfates、glucuronides等。研究結果顯示，國人飲用三種富含黃酮的飲料後，從尿液排除之百分率為豆漿>葡萄柚汁>柳橙汁，排除半生期為豆漿>葡萄柚汁>柳橙汁，顯示豆漿中的黃酮吸收較佳，且其代謝物於體內滯留時間較長。葡萄柚汁、柳橙汁、豆漿中黃酮代謝物排出最多之時段分別為8-10、4-6及8-10小時。本研究所建立之尿液分析方法，可提供國人未來進行營養流行病學調查研究之方法，並初步建立國人異黃酮相關微營養素吸收代謝之本土資料，應為日後以營養教育介入慢性病防治之重要實證依據。 Many multi-national epidemiological studies revealed that daily intake of flavonoids was correlated with the incidences of cardiovascular diseases, cancers and neurodegenerative diseases. Therefore, flavonoids are considered as a group of micronutrients. This study established simple and precise HPLC/UV methods to analyze flavonoids in human urine. After healthy volunteers received native beverages including grapefruit juice, orange juice and soymilk, glycosides and aglycones of flavonoids including naringenin, hesperetin, genistein, daidzein and equol as well as their glucuronides and sulfates in the urine samples were determined before and after hydrolysis with ？H]-glucuronidase and sulfatase, respectively. Our results showed that the decreasing order of urinary excretion percentages of flavonoids were soymilk > grapefruit juice > orange juice, indicating that soymilk isoflavones have the best absorption. Moreover, the decreasing order of half-lives were also soymilk >grapefruit juice >orange juice, indicatng that soymilk isoflavones have residence time in the body. Given the bioactivities of the flavonoid conjugated metabolites from these three beverages were comparable, soymilk would be the best resource of micronutrients than grapefruit juice and orange juice. The highest excretion intervals of flavoinoid sulfates/glucuronides after taking grapefruit juice, orange juice and soymilk were 8-10, 4-6 and 8-10 h, respectively. This study could provide basic information for the data base of micronutrients. The analysis method established in this study could be applicable for the nutrition status survey in the future.
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