雞副嗜血桿菌(Haemophilus paragallinarum)A 型及C 型菌株不同致病因子之搜尋

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题名:	雞副嗜血桿菌(Haemophilus paragallinarum)A 型及C 型菌株不同致病因子之搜尋
作者:	徐媛曼(Hsu,Yuan-Man);陳翰民
贡献者:	醫學院生物科技學系
关键词:	雞副嗜血桿菌;傳染性可利查;聚合脢鏈鎖反應基礎之扣除雜交法;二維電泳;Haemophilus paragallinarum;infectious coryza;PCR-based subtractive hybridization method;2-Dimensional gel electrophoresis
日期:	2006-07-31
上传时间:	2009-09-01 15:51:49 (UTC+8)
摘要:	傳染性可利查(Infectious coryza, IC)，俗稱傳染性鼻炎，是由雞副嗜血桿菌所引起(Haemophilus paragallinarum)，本病是一世界性傳染性的雞重要疾病.由於其發病率相當高，且多發生在成長中或產蛋雞群，造成死亡及產蛋率暴跌 (10-40%)的嚴重損失，對雞農造成的損失很大。本病僅感染雞，公共衛生上毫無影響，但為台灣細菌性禽病中最為困擾的問題之一。目前非活化性疫苗與抗生素雖廣為使用，但其控制效果仍然有限。由於疫苗製備的過程中，菌株之生長環境與其表面抗原的表現量及種類有密切的關係，因此增加了疫苗製備的困難。也因為此菌的致病機轉仍不清楚，增加診斷此菌血清型的困難。因此，本研究將針對台灣流行的V-factor-dependent H. paragallinarum血清型A及C做系統性的比較，得到兩個僅在血清型A出現之蛋白質，hctC與putative transposase；並且在蛋白圖譜的表現上，hemagglutinin antigen與OmpA 在含鐵的環境中於兩個血清型中表現量均較高。此項研究工作之完成將可有助於了解H. paragallinarum的分子機轉，並進一步改善H.paragallinarum的疫苗效益，不同血清型間的交叉保護作用及更方便的血清型檢測之方式，對H. paragallinarum在台灣引起之傳染性可利查(Infectious Coryza, IC)的防治及診斷有實質上的效益。 Infectious coryza, an acute upper respiratory tract disease of chickens, is caused by the bacterium Haemophilus paragallinarum. This disease is of worldwide economic significance and affects both broiler and layer flocks, manifesting primarily as a drop in egg production (10-40%) in layer flocks and retardation of growth. The chicken is the natural host of H. paragallinarum, and it is the one of the most serious bacterial infection in poultry. Even though the inactivated vaccines and antibiotics are wildly used to treat or prevent an outbreak of this disease, neither of them is effective. The difficulty of perpetrating inactivated vaccines is that many different factors, mainly growth conditions, appear to influence the expression of the antigens of H. paragallinarum. The pathogenesis of H. paragallinarum is still unclear, therefore, it increases the difficulty of diagnosis which serogroup causes infection in poultry. In this study, we examed Taiwan isolated H. paragallinarum serogroups A and C by PCR-based subtractive hybridization method and 2-Dimensional gel electrophoresis. By subtractive hybridization analysis, there were two genes , hctC and putative transposase, were specific for serogroup A; by 2-Dimensional gel electrophoresis analysis, hemagglutinin antigen and OmpA expressed more in the iron-limited condition in both serogroups A and C. By doing so, we will be able to increase the efficiency of vaccines and differential diagnosis in infection.
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